{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:22:10Z","timestamp":1759778530026},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,10]],"date-time":"2015-06-10T00:00:00Z","timestamp":1433894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81171798","2014ZD004","81421064","81230041"],"award-info":[{"award-number":["81171798","2014ZD004","81421064","81230041"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7142124"],"award-info":[{"award-number":["7142124"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:title>Background\/Purpose<\/jats:title><jats:p>The monitoring of autofluorescence in skin tissue samples can have diagnostic and therapy significance. In this study, we are the first to describe autofluorescence of eccrine sweat glands, which is important and helpful for the diagnosis and therapy of diseases that involve the eccrine sweat glands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eccrine sweat gland autofluorescence in haematoxylin\u2010eosin (<jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>) stained skin tissue sections was observed under a fluorescence microscope, which was compared to the immunofluorescence of keratin 19 and 15 in the skin tissue sections. The single eccrine sweat glands from five volunteers including three males and two females were isolated and also observed under a fluorescence microscope. The autofluorescence intensity of the single eccrine sweat gland was measured using a laser confocal scanning microscope system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eccrine sweat gland autofluorescence in <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> stained skin tissue sections appears green under <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> fliter system (470\/40\u00a0nm) and red under N2.1 fliter system (515\u2013560\u00a0nm). Furthermore, the single eccrine sweat gland showed various autofluorescence colours, including green under wide blue and red under wide green. The autofluorescence intensity of the single eccrine sweat gland was measured. The spectrum excited at 488\u00a0nm exhibited two peaks located at approximately 530\u00a0nm (11.54\u00a0\u00b1\u00a04.66) and 590\u00a0nm (10.38\u00a0\u00b1\u00a04.33). The results suggest flavin and lipopigment as the endogenous fluorophores.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The autofluorescence of the <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> stained eccrine sweat gland sections is simple and helpful for easily determining the structure of eccrine sweat glands. The autofluorescence of the single eccrine sweat gland may be due to the existence of flavin and lipopigment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12234","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T15:13:20Z","timestamp":1434726800000},"page":"98-103","source":"Crossref","is-referenced-by-count":6,"title":["Autofluorescence of eccrine sweat glands"],"prefix":"10.1111","volume":"22","author":[{"given":"HL.","family":"Zhao","sequence":"first","affiliation":[{"name":"General Hospital of the Chinese People's Liberation Army Beijing China"},{"name":"The First Hospital of Shijiazhuang Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy General Hospital of Beijing Military Region DongCheng District Beijing 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