{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T11:18:47Z","timestamp":1721215127578},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T00:00:00Z","timestamp":1468800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tokiwa Pharmaceutical Co., Ltd"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"Background\/Purpose<\/jats:title>Skin melanin content is an important indicator for ascertaining the pathology of skin pigmentation diseases, but its analysis necessitates a biopsy or other means of collecting tissue, posing a considerable burden to the patient, and making it difficult to observe how a given skin site changes over time. Here, we aimed to establish a non\u2010invasive method for quantifying the eumelanin and pheomelanin content of the stratum corneum.<\/jats:p><\/jats:sec>Methods<\/jats:title>Sun\u2010exposed and non\u2010exposed samples from 10 healthy Japanese subjects were compared. We harvested the outermost layer of the stratum corneum by tape\u2010stripping, considering the outer side of the forearm as a sun\u2010exposed area, and medial side of the upper arm as a non\u2010exposed area. Four additional subjects were included in the analysis of change in melanin content over time at the same skin site. The anterior lower leg received a single exposure to two minimal erythema dose sunlight, and the stratum corneum was harvested from the same site over a period of 20 weeks; we subsequently quantified the levels of eumelanin and pheomelanin using high\u2010performance liquid chromatography.<\/jats:p><\/jats:sec>Results<\/jats:title>We were able to accurately quantify the eumelanin and pheomelanin contents of the stratum corneum, and to observe the evolution of the same skin site over time. Eumelanin levels were significantly higher in the sun\u2010exposed area, with a peak in melanin observed after 11\u201315 weeks of sun exposure.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>This non\u2010invasive method can serve as a marker for pathology of skin pigmentation diseases such as malignant tumors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12309","type":"journal-article","created":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T11:29:10Z","timestamp":1468841350000},"page":"104-111","source":"Crossref","is-referenced-by-count":7,"title":["Non\u2010invasive quantification of melanin in the stratum corneum: a novel indicator of skin lesions in pigmentation diseases"],"prefix":"10.1111","volume":"23","author":[{"given":"H.","family":"Matsunaka","sequence":"first","affiliation":[{"name":"Department of Dermatology Wakayama Medical University Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology Wakayama Medical University Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Furukawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Wakayama Medical University Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2008.00455.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.146.s61.3.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74439-1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140402.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1995.tb09883.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.1979.tb01931.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008390-199710000-00005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2012.01011.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1996.tb02258.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2003.00072.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02361.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12410"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-6649rev"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411960070040101"}],"container-title":["Skin Research and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsrt.12309","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsrt.12309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/srt.12309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T11:20:06Z","timestamp":1695554406000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/srt.12309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,18]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/srt.12309"],"URL":"https:\/\/doi.org\/10.1111\/srt.12309","archive":["Portico"],"relation":{},"ISSN":["0909-752X","1600-0846"],"issn-type":[{"value":"0909-752X","type":"print"},{"value":"1600-0846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,18]]}}}