{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:36:52Z","timestamp":1789385812997,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006209","name":"Radboud Universitair Medisch Centrum","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Background\/Purpose<\/jats:title><jats:p>FibroTx Transdermal Analyses Patch (<jats:styled-content style=\"fixed-case\">TAP<\/jats:styled-content>) is a novel technology for non\u2010invasive measurements of protein biomarkers on the skin surface, <jats:italic>in vivo<\/jats:italic>. The aim of this study was to explore the potential of <jats:styled-content style=\"fixed-case\">TAP<\/jats:styled-content> in detecting skin surface biomarkers following mild perturbations, <jats:italic>in vivo<\/jats:italic>, using two experimental models: tape stripping, mimicking acute barrier disruption, and histamine iontophoresis, mimicking acute and local inflammation at minimal skin barrier insult.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tape stripping and histamine iontophoresis were performed in two separate experiments on the volar forearm of healthy volunteers (<jats:italic>n<\/jats:italic> = 27 and <jats:italic>n<\/jats:italic> = 10, respectively). Biomarker levels were assessed with <jats:styled-content style=\"fixed-case\">TAP<\/jats:styled-content> at baseline and up to 72 h after stimulation. Functional (transepidermal water loss \u2013<jats:styled-content style=\"fixed-case\">TEWL<\/jats:styled-content>\u2013 and a* value) and morphological (confocal reflectance microscopy \u2013<jats:styled-content style=\"fixed-case\">RCM<\/jats:styled-content>) assessments were added in the tape stripping and histamine iontophoresis experiments, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cytokines <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b1 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content> and the antimicrobial peptide <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 showed distinct dynamics, despite substantial inter\u2010individual variation in levels, with an increase following tape stripping and a decrease following histamine iontophoresis. These dynamics could be related to the assessments made by <jats:styled-content style=\"fixed-case\">TEWL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RCM<\/jats:styled-content>. In the tape stripping experiment, additional biomarkers could be detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TAP<\/jats:styled-content> measurements, especially <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101, from the skin surface were sensitive enough for monitoring dynamic changes in the skin in the two models of skin perturbation. We conclude that <jats:styled-content style=\"fixed-case\">TAP<\/jats:styled-content> holds promise for non\u2010invasively unraveling the dynamics of processes related to skin perturbation and repair.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12340","type":"journal-article","created":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T05:07:21Z","timestamp":1477976841000},"page":"336-345","source":"Crossref","is-referenced-by-count":16,"title":["Measurement of skin surface biomakers by Transdermal Analyses Patch following different <i>in vivo<\/i> models of irritation: a pilot study"],"prefix":"10.1111","volume":"23","author":[{"given":"D.","family":"Falcone","sequence":"first","affiliation":[{"name":"Department of Dermatology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Spee","sequence":"additional","affiliation":[{"name":"FibroTX LLC Tallinn Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Salk","sequence":"additional","affiliation":[{"name":"FibroTX LLC Tallinn Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Peppelman","sequence":"additional","affiliation":[{"name":"Department of Dermatology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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