{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:22:18Z","timestamp":1790266938720,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,4]],"date-time":"2019-12-04T00:00:00Z","timestamp":1575417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Royal Aage Bangs Foundation"},{"name":"Hospital Region H Research Foundation"},{"name":"Hans and Nora Buchards Foundation"},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"crossref","award":["NNF15OC0017452"],"award-info":[{"award-number":["NNF15OC0017452"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Black tattoo reactions are suggested to be foreign body reactions manifested as papulo\u2010nodular inflammation that is associated with active sarcoidosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To study the morphology of black and red pigment agglomerates in skin biopsies from tattoo reactions, referenced to clinical diagnoses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Agglomerate count, area, width, height, circumference and circularity were measured in 161 patients by light microscopy (Olympus BX51\u2122); 161 unstained skin biopsies from 64 black tattoo reactions and 97 reactions in red tattoos with allergy. Images (Jenoptic Gryphax RGB camera) were taken through a 40\u00d7 objective using immersion oil. Computerised analysis of agglomerates was performed using ImageJ software. Student<jats:italic>t<\/jats:italic>test, chi\u2010square test, Fisher Exact test, ANOVA and Bonferroni tests were applied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparison of black and red pigment agglomerates showed no overall differences in count, area, width, height or circumference. However, in black tattoo reactions, the count of agglomerates was higher in patients with sarcoidosis (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.009) vs no sarcoidosis. Black agglomerates were more circular shaped as compared to red,<jats:italic>P<\/jats:italic>\u00a0=\u00a0.023. In red tattoo reactions, agglomerates in patients with allergic cross\u2010reactivity were slightly more circular (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.036) vs patients with milder allergy. The count of red agglomerates was lower in patients clinically typed excessive hyperkeratosis (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.041) compared with other clinical types of allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Patients with reactions in black tattoos associated with sarcoidosis have increased count of pigment agglomerates vs those without associated sarcoidosis indicating that black pigment agglomeration is one among a number of factors triggering sarcoid tattoo complication. Circular shape of agglomerates may code for bioactivity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12798","type":"journal-article","created":{"date-parts":[[2019,12,4]],"date-time":"2019-12-04T13:22:02Z","timestamp":1575465722000},"page":"284-291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Tattoo pigment agglomerates measured in skin biopsies by computerised light microscopy"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4183-0580","authenticated-orcid":false,"given":"Katrina","family":"Hutton Carlsen","sequence":"first","affiliation":[{"name":"Department of Dermatology The \u201cTattoo Clinic\u201d Bispebjerg University Hospital Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Larsen","sequence":"additional","affiliation":[{"name":"Bio\u2010Science ApS Gilleleje Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen","family":"Serup","sequence":"additional","affiliation":[{"name":"Department of Dermatology The \u201cTattoo Clinic\u201d Bispebjerg University Hospital Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/isbn.978-3-318-02777-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/isbn.978-3-318-05978-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16040"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.4103\/ijd.IJD_210_16","article-title":"Hypersensitive reaction to tattoos: a growing menace in rural India","volume":"62","author":"Shashikumar BM","year":"2017","journal-title":"Indian J Dermatol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/GOX.0000000000001111"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12425"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000453315"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.20175860"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3889\/oamjms.2018.049"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14854"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.5070\/D31FM1D840","article-title":"Sarcoidal tattoo granuloma","volume":"16","author":"Anolik R","year":"2010","journal-title":"Dermatol Online J"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000452148"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12271"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","unstructured":"DirkW.Primary particles \u2010 agglomerates \u2010 aggregates. 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