{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:43:19Z","timestamp":1773812599321,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Enteric neuropathy is described in most patients with gastrointestinal dysmotility and may be found together with reduced intraepidermal nerve fiber density (IENFD). The aim of this pilot study was to assess whether three\u2010dimensional (3d) imaging of skin biopsies could be used to examine various tissue components in patients with gastrointestinal dysmotility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Four dysmotility patients of different etiology and two healthy volunteers were included. From each subject, two 3\u2010mm punch skin biopsies were stained with antibodies against protein gene product 9.5 or evaluated as a whole with two X\u2010ray phase\u2010contrast computed tomography (CT) setups, a laboratory \u00b5CT setup and a dedicated synchrotron radiation nanoCT end\u2010station.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two patients had reduced IENFD, and two normal IENFD, compared with controls. \u00b5CT and X\u2010ray phase\u2010contrast holographic nanotomography scanned whole tissue specimens, with optional high\u2010resolution scans revealing delicate structures, without differentiation of various fibers and cells. Irregular architecture of dermal fibers was observed in the patient with Ehlers\u2010Danlos syndrome and the patient with idiopathic dysmotility showed an abundance of mesenchymal ground substance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>3d phase\u2010contrast tomographic imaging may be useful to illustrate traits of connective tissue dysfunction in various organs and to demonstrate whether disorganized dermal fibers could explain organ dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.12974","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T22:08:59Z","timestamp":1602022139000},"page":"316-323","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["3d phase\u2010contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between 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Radiation Physics Department of Clinical Sciences Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabet","family":"Englund","sequence":"additional","affiliation":[{"name":"Division of Oncology and Pathology Skane University Hospital Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9la","family":"Veress","sequence":"additional","affiliation":[{"name":"Department of Pathology Sk\u00e5ne University Hospital Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Salditt","sequence":"additional","affiliation":[{"name":"Institute for X\u2010Ray Physics University of G\u00f6ttingen G\u00f6ttingen Germany"},{"name":"Cluster of Excellence \"Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells\" (MBExC) University of G\u00f6ttingen G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars 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