{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:26:09Z","timestamp":1767849969844,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the expression of interleukin\u20101\u03b2 (IL\u20101\u03b2), interleukin\u20104 (IL\u20104), interferon\u2010\u03b3 (IFN\u2010\u03b3) and tumour necrosis factor \u03b1 (TNF\u2010\u03b1) in different tissue in a dinitrochlorobenzene (DNCB)\u2010induced ear swelling test in mice and further evaluate the correlation between the cytokine expression in different tissues and the degree of ear swelling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The mice were sensitised with a 0.50% DNCB solution on their back for 3\u00a0days. After 7 days, the thickness of their ears was measured and grouped. Different concentrations of the DNCB solution were challenged in the left ear of each group of mice, and the right ear was used as the control. The thickness of both ears was measured every 24\u00a0h, and the mice were sacrificed 72\u00a0h after the challenge. The expressions of IL\u20101\u03b2, IL\u20104, IFN\u2010\u03b3 and TNF\u2010\u03b1 in the mouse serum, lymph node and ear tissue were quantified by enzyme\u2010linked immunosorbent assay, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was a linear positive correlation between the swelling index of the mouse lateral ear and the challenge concentration of DNCB (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.96,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01). The high expression of IL\u20101\u03b2 and IL\u20104 in the lateral ear tissue of the mice was positively correlated with the ear swelling index 48\u00a0h after the challenge. The correlation coefficient was 0.78 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01). Furthermore, IFN\u2010\u03b3 and TNF\u2010\u03b1 had no significant correlation with the ear swelling index 48\u00a0h after the challenge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There is a correlation between the degree of ear swelling in mice and the concentration of DNCB and the expression of IL\u20101\u03b2 and IL\u20104 in the lateral ear tissue. There is a sub\u2010clinical skin sensitivity state in contact allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.13255","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T07:59:45Z","timestamp":1671695985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expression of interleukin\u20101\u03b2, interleukin\u20104, interferon\u2010\u03b3 and tumour necrosis factor \u03b1 in different tissue in a dinitrochlorobenzene\u2010induced ear swelling test in mice"],"prefix":"10.1111","volume":"29","author":[{"given":"Chun\u2010Lian","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology Shijingshan teaching hospital of Capital Medical University, Beijing Shijingshan Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2007.06.009"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9010003"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2021.112331"},{"issue":"2020","key":"e_1_2_11_5_1","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/4307161","article-title":"Quercitrin, the main compound in wikstroemia indica, mitigates skin lesions in a mouse model of 2,4\u2010dinitrochlorobenzene\u2010induced contact hypersensitivity","volume":"9","author":"Jegal J","year":"2020","journal-title":"Evid Based Complement Alternat Med"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-018-2164-5"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201019666181029111011"},{"issue":"3","key":"e_1_2_11_8_1","first-page":"145","article-title":"Construction of rats model for allergic contact dermatitis induced by DNCB","volume":"31","author":"He WF","year":"2015","journal-title":"China journal of Leprosy and Skin Diseases"},{"issue":"11","key":"e_1_2_11_9_1","first-page":"848","article-title":"The dose\u2010effect of light concentration DNCB in inducing guinea pig sub\u2010clinical contact dermatitis","volume":"25","author":"Li CL","year":"2011","journal-title":"The Chinese Journal of Dermatovenereology"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13659"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2010.12.007"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2011.02832.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.986943"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"issue":"3","key":"e_1_2_11_15_1","first-page":"439","article-title":"Induction effect of DNCB on atopic dermatitis BALB\/c mice and its mechanism","volume":"46","author":"Wang XX","year":"2020","journal-title":"Journal of Jilin University"},{"issue":"7","key":"e_1_2_11_16_1","first-page":"605","article-title":"Characteristics of skin microbiota in an atopic dermatitis\u2010like mouse model induced by 2,4\u2010dinitrochlorobenzene","volume":"54","author":"Men YH","year":"2021","journal-title":"Chinese Journal of Dermatology"},{"issue":"8","key":"e_1_2_11_17_1","first-page":"1314","article-title":"Influence of PVL and PDTC on IL\u20101\u03b2 concentration in THP\u20101","volume":"17","author":"Xie Q","year":"2013","journal-title":"Anhui Medicine"},{"issue":"2","key":"e_1_2_11_18_1","first-page":"138","article-title":"Therapeutic effect and discussion of immunological mechanism of compound lidocaine cream on contact dermatitis model of mice","volume":"27","author":"Sheng GR","year":"2013","journal-title":"The Chinese Journal of Dermatovenereology"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-012-8277-7"}],"container-title":["Skin Research and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/srt.13255","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/srt.13255","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/srt.13255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T21:43:43Z","timestamp":1728596623000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/srt.13255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,21]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/srt.13255"],"URL":"https:\/\/doi.org\/10.1111\/srt.13255","archive":["Portico"],"relation":{},"ISSN":["0909-752X","1600-0846"],"issn-type":[{"value":"0909-752X","type":"print"},{"value":"1600-0846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,21]]},"assertion":[{"value":"2022-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13255"}}