{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T02:04:27Z","timestamp":1773021867464,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T00:00:00Z","timestamp":1692662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pyroptosis has been implicated in the development of human diseases, including vitiligo. TanshinoneIIA has been confirmed to play anti\u2010vitiligo role. However, whether tanshinoneIIA inhibits vitiligo progression via regulating cell pyroptosis remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>)\u2010induced melanocytes were used to mimic vitiligo cell model in vitro. Cell viability was assessed by cell counting kit 8 assay, and reactive oxygen species (ROS) production was detected by DCFH\u2010DA staining. Nod\u2010like receptor protein 3 (NLRP3) expression was detected by quantitative real\u2010time PCR, western blot and immunofluorescence staining. Cell pyroptosis was measured using flow cytometry, and the contents of interleukin\u20101\u03b2 and interleukin\u201018 were determined by ELISA. Besides, the protein levels of apoptosis\u2010associated speck\u2010like protein containing CARD\u00a0(ASC) and cleaved\u2010Caspase\u20101 were examined by western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> could induce ROS production, NLRP3 expression and pyroptosis in melanocytes. TanshinoneIIA inhibited ROS production, pyroptosis, and the expression of NLRP3, ASC and cleaved\u2010caspase\u20101 in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced melanocytes. Compared with the function of ROS inhibitor (NAC), tanshinoneIIA acted as a ROS scavenger to relieve melanocyte pyroptosis. In addition, NLRP3 inhibitor (MCC950) also could aggravate the inhibition effect of tanshinoneIIA on melanocyte pyroptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TanshinoneIIA suppressed melanocyte pyroptosis probably through modulating the ROS\/NLRP3 signaling axis, which provides the evidence for therapeutic effect in vitiligo.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.13419","type":"journal-article","created":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T08:16:28Z","timestamp":1692692188000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["TanshinoneIIA inhibits melanocyte pyroptosis by regulating the ROS\/NLRP3 signaling axis"],"prefix":"10.1111","volume":"29","author":[{"given":"Xiaosha","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha City P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyang","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine Hunan University of Chinese Medicine Changsha City P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haizhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha City P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Diagnostic in Chinese Medicine Hunan University of Chinese Medicine Changsha City P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-100919-023531"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.15743"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728214.2020.1712358"},{"issue":"6","key":"e_1_2_9_5_1","first-page":"688","article-title":"Beyond traditional treatment: the importance of psychosocial therapy in vitiligo","volume":"17","author":"Rzepecki AK","year":"2018","journal-title":"J Drugs Dermatol"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2022.2123864"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8498472"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00536"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.10.036"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.62521"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2020.06.044"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-021-00740-6"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1761-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-022-03566-6"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0891-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S266911"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114595"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.15086"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.30398"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.152"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.914898"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2015.09.003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.05.010"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S114328"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108599"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000505493"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2017.08.059"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2022.2074619"},{"issue":"5","key":"e_1_2_9_29_1","first-page":"3140","article-title":"Tanshinone IIA ameliorates oxaliplatin\u2010induced neurotoxicity via mitochondrial protection and autophagy promotion","volume":"11","author":"Cheng W","year":"2019","journal-title":"Am J Transl Res"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4991"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvz198"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000024117"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202202"}],"container-title":["Skin Research and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/srt.13419","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T07:49:26Z","timestamp":1753170566000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/srt.13419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,22]]},"references-count":32,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/srt.13419"],"URL":"https:\/\/doi.org\/10.1111\/srt.13419","archive":["Portico"],"relation":{},"ISSN":["0909-752X","1600-0846"],"issn-type":[{"value":"0909-752X","type":"print"},{"value":"1600-0846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,22]]},"assertion":[{"value":"2023-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13419"}}