{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:22:27Z","timestamp":1770834147813,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T00:00:00Z","timestamp":1707350400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373485"],"award-info":[{"award-number":["82373485"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872529"],"award-info":[{"award-number":["81872529"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Psoriasis is a prevalent, long\u2010term skin condition characterized by inflammation. Keratinocytes (KCs) are important effector cells that release inflammatory factors and chemokines to promote the inflammatory cascade in psoriasis. However, the mechanisms underlying the activation of KCs in psoriasis remain unclear. Livin suppresses apoptotic proteins and directly affects the growth and spread of cancer cells. Livin expression reportedly increases significantly in lesions of patients with psoriasis; however, its specific role in KC activation remains unknown. This study aimed to examine the impact of Livin on KC activation and the subsequent release of inflammatory mediators.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunofluorescence staining, reverse transcription\u2010quantitative polymerase chain reaction, enzyme\u2010linked immunosorbent assay (ELISA), and western blotting were used to assess Livin expression in patients with psoriasis, an imiquimod (IMQ)\u2010induced psoriasis\u2010like mouse model, and M5\u2010treated HaCaT cells. To investigate the role of Livin in KCs, we performed RNA sequencing and proteomic analysis of Livin\u2010knockdown (knockdown\u2010HaCaT) and negative control (NC\u2010HaCaT) cells. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were used for enrichment analyses. Moreover, the effect of Livin expression on the release of inflammatory mediators in KCs was verified using ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Livin expression was higher in KCs of patients with psoriasis than in those healthy controls. Livin expression in HaCaT cells treated with M5 increased significantly over time. Livin expression was higher in the skin lesions of the IMQ mouse model than in the control group. Proteomic analysis and RNA sequencing used to investigate the function of Livin in HaCaT cells revealed its potential role in mediating KC activation and inflammatory mediator release, which affected the pathology of psoriasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Livin expression played an effect on KCs activation, which induced release of inflammatory mediators and up\u2010regulation of keratin. This study provides a new effector molecule for the mechanism of inflammatory response in psoriasis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.13603","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T00:31:31Z","timestamp":1707438691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis"],"prefix":"10.1111","volume":"30","author":[{"given":"Delu","family":"Che","sequence":"first","affiliation":[{"name":"Department of Dermatology Xi'an Jiaotong University Second Affiliated Hospital (Xibei Hospital)  Xi'an China"},{"name":"Center for Dermatology Disease Precision Medical Institute  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yazhuo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xi'an Jiaotong University Second Affiliated Hospital (Xibei Hospital)  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Hang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xi'an Jiaotong University Second Affiliated Hospital (Xibei Hospital)  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaili","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xi'an Jiaotong University Second Affiliated Hospital (Xibei Hospital)  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaijie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology the 1st affiliated hospital, Jiangxi Medical College Nanchang University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Xi'an Jiaotong University Second Affiliated Hospital (Xibei Hospital)  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32549-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.714274"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222112035"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.222"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.01.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.20.8577"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2013.1925"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14768"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP088584"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14139"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-018-0505-z"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06116"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03963"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14445"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-08-0480"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmp118"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2017.5584"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116788"},{"issue":"11","key":"e_1_2_10_20_1","first-page":"14034","article-title":"Expression 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