{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:11:25Z","timestamp":1781197885195,"version":"3.54.1"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T00:00:00Z","timestamp":1710115200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Current methods for evaluating efficacy of cosmetics have limitations because they cannot accurately measure changes in the dermis. Skin sampling using microneedles allows identification of skin\u2010type biomarkers, monitoring treatment for skin inflammatory diseases, and evaluating efficacy of anti\u2010aging and anti\u2010pigmentation products.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>Two studies were conducted: First, 20 participants received anti\u2010aging treatment; second, 20 participants received anti\u2010pigmentation treatment. Non\u2010invasive devices measured skin aging (using high\u2010resolution 3D\u2010imaging in the anti\u2010aging study) or pigmentation (using spectrophotometry in the anti\u2010pigmentation study) at weeks 0 and 4, and adverse skin reactions were monitored. Skin samples were collected with biocompatible microneedle patches. Changes in expression of biomarkers for skin aging and pigmentation were analyzed using qRT\u2010PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>No adverse events were reported. In the anti\u2010aging study, after 4 weeks, skin roughness significantly improved in 17 out of 20 participants. qRT\u2010PCR showed significantly increased expression of skin\u2010aging related biomarkers: PINK1 in 16\/20 participants, COL1A1 in 17\/20 participants, and MSN in 16\/20 participants. In the anti\u2010pigmentation study, after 4 weeks, skin lightness significantly improved in 16\/20 participants. qRT\u2010PCR showed significantly increased expression of skin\u2010pigmentation\u2010related biomarkers: SOD1 in 15\/20 participants and Vitamin D Receptor (VDR) in 15\/20 participants. No significant change in TFAP2A was observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Skin sampling and mRNA analysis for biomarkers provides a novel, objective, quantitative method for measuring changes in the dermis and evaluating the efficacy of cosmetics. This approach complements existing evaluation methods and has potential application in assessing the effectiveness of medical devices, medications, cosmeceuticals, healthy foods, and beauty devices.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.13647","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T06:13:40Z","timestamp":1710137620000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Microneedles: A novel clinical technology for evaluating skin characteristics"],"prefix":"10.1111","volume":"30","author":[{"given":"Seo Hyeong","family":"Kim","sequence":"first","affiliation":[{"name":"Cutis Biomedical Research Center Co. Ltd.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hye","family":"Kim","sequence":"additional","affiliation":[{"name":"Cutis Biomedical Research Center Co. Ltd.  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