{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:45:18Z","timestamp":1787201118457,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T00:00:00Z","timestamp":1713312000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Reconstructed epidermis models, obtained from 3D keratinocytes culture, have gained significant prominence as prototypes for safety and efficacy testing in skin research. To effectively evaluate these models, it is essential to perform molecular and functional characterization. The skin's barrier function is one of the essential aspects of the epidermis that needs to be assessed. A noninvasive method is thus required for the evaluation of the skin barrier in these models. With this perspective, the aim of this feasibility study is to apply the speckle technique for the assessment of the skin barrier in the Reconstructed Human Epidermis (RHE).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>Speckle analysis as well as Raman microspectroscopy were performed on RHE samples at two maturation days, D17 and D20.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Between D17 and D20, our study showed an increase in various Raman parameters, including stratum corneum percentage, lateral lipid packing, lipid\u2010to\u2010protein ratio, and protein secondary structure. Furthermore, the degree of light polarization and the speckle grain size also increased over this period.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The speckle technique proved to be effective for evaluating the skin barrier in Reconstructed Human Epidermis (RHE) models. Comparison with Raman validates this approach and provides comprehensive molecular and functional characterization of reconstructive skin models.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.13708","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T01:54:10Z","timestamp":1713405250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Noninvasive evaluation of the skin barrier in reconstructed human epidermis using speckle analysis: Correlation with Raman microspectroscopy"],"prefix":"10.1111","volume":"30","author":[{"given":"L\u00e9a","family":"Habib","sequence":"first","affiliation":[{"name":"Laboratoire d\u2019\u00e9tude cin\u00e9tique en milieu h\u00e9t\u00e9rog\u00e8ne (LECH) Facult\u00e9 des Sciences Universit\u00e9 Saint Joseph  Beirut Lebanon"},{"name":"Unit\u00e9 Universitaire Interdisciplinaire\u00a0Lip(Sys)<sup>2<\/sup>: Lipides, Syst\u00e8mes analytiques et biologiques, Facult\u00e9 de pharmacie Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a Abi","family":"Nassif","sequence":"additional","affiliation":[{"name":"Physics Department, UR TVA, Facult\u00e9 des sciences Universit\u00e9 Saint Joseph  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Abboud","sequence":"additional","affiliation":[{"name":"Physics Department, UR TVA, Facult\u00e9 des sciences Universit\u00e9 Saint Joseph  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rime","family":"Michael\u2010Jubeli","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Universitaire Interdisciplinaire\u00a0Lip(Sys)<sup>2<\/sup>: Lipides, Syst\u00e8mes analytiques et biologiques, Facult\u00e9 de pharmacie Universit\u00e9 Paris\u2010Saclay  Orsay 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biosynth\u00e8se lipidique au cours de la diff\u00e9renciation k\u00e9ratinocytaire en lien avec l'int\u00e9grit\u00e9 de la fonction barri\u00e8re cutan\u00e9e","author":"Bakar J","year":"2021"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2020.113606"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2014.02.014"},{"key":"e_1_2_9_6_1","unstructured":"SkinEthicTM RHE \/Reconstructed Human Epidermis.https:\/\/www.episkin.com\/SkinEthic\u2010RHE#:~:text=SkinEthicTM%20RHE%20is%20an 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