{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:20:46Z","timestamp":1788999646075,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acne vulgaris (AV) is a chronic inflammatory skin condition affecting the pilosebaceous unit, commonly presenting as comedones, papules, pustules, or nodules on the face, upper limbs, torso, and back, with comedones formation being the primary pathology leading to disfiguring inflammation, hyperpigmentation, scarring, and psychological impact.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The purpose of this study was to investigate the significance of two genetic variants in the promoter region of the tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) gene and their association with insulin resistance (IR) in acne patients. To understand how these variants contribute to AV and its associated IR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and methods<\/jats:title>\n                    <jats:p>An analytical cross\u2010sectional study with a case\u2010control design and research evaluation was carried out on 87 AV patients and 73 healthy volunteers. The medical histories of both groups were obtained, as well as the severity and duration of inflammation among acne sufferers, as well as demographic data. Biochemical analysis was performed on both sets of participants, including fasting blood glucose levels, insulin levels while fasting, IR, and serum TNF\u2010\u03b1. PCR\u2010RFLP analysis identified \u2212863 G\u00a0&gt;\u00a0A (rs1800630) and \u2212308 G\u00a0&gt;\u00a0A (rs1800629) variations, and real\u2010time PCR analysis evaluated TNF\u2010\u03b1 gene expression in both patients and healthy people.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Acne patients exhibited significantly higher levels of IR, fasting glucose, fasting insulin, serum TNF\u2010\u03b1, and TNF\u2010\u03b1 folding change, when compared to healthy controls. The co\u2010dominant model for \u2212863 G\u00a0&gt;\u00a0A and \u2212308 G\u00a0&gt;\u00a0A variants exhibited significant variations between the two groups. Severe acne patients who had the A\/A genotype for \u2212308 variants exhibited higher levels of IR, serum TNF\u2010\u03b1, and TNF\u2010\u03b1 folding change. Highly significant positive linear correlation between IR, serum TNF\u2010\u03b1, and TNF\u2010\u03b1 folding change in severe AV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>There is a correlation between AV, especially severe acne, and the \u2212863 G\u00a0&gt;\u00a0A and \u2212308 G\u00a0&gt;\u00a0A polymorphism, which influences TNF\u2010\u03b1 gene expression and serum TNF\u2010\u03b1 levels.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.13811","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T08:09:28Z","timestamp":1719302968000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["In severe acne vulgaris, TNF\u2010\u03b1 gene variants are connected to increased TNF\u2010\u03b1 gene expression and insulin resistance"],"prefix":"10.1111","volume":"30","author":[{"given":"Ahmed Ibrahim","family":"AbdElneam","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Department of Basic Medical Sciences College of Medicine Shaqra University  Dawadmi Saudi Arabia"},{"name":"Molecular Genetics and Enzymology Department Human Genetics and Genome Research Institute National Research Center  Dokki Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharif","family":"Alhajlah","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratories College of Applied Medical Sciences Shaqra University  Shaqra Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Saleh","family":"Al\u2010Dhubaibi","sequence":"additional","affiliation":[{"name":"Departments of Dermatology College of Medicine Shaqra University  Dawadmi Saudi 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Mohammed","family":"Atef","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Sexology Faculty of Medicine Suez Canal University  Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"StatPearls [Internet]","author":"Sutaria 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