{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T10:04:15Z","timestamp":1781517855628,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["PAPIIT IN117520"],"award-info":[{"award-number":["PAPIIT IN117520"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Wound healing monitoring and timely decision\u2010making are critical for wound classification. Tryptophan (Tr) intrinsic fluorescence, detected at 295\/340\u00a0nm, provides a noninvasive approach for wound assessment. Our previous work demonstrated that this autofluorescence is associated with keratinocytes in a highly proliferative state in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We investigated the correlation between Tr fluorescence and key wound healing parameters, including re\u2010epithelialization, fibrosis, neovascularization, and acute and chronic inflammation, using a rabbit model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Seven rabbits underwent wound healing assessment over a 15\u2010day period. We employed histological analysis from central and marginal biopsies, and UV fluorescence imaging captured by a monochromatic near\u2010UV sensitive camera equipped with a passband optical filter (340\u00a0nm\/12\u00a0nm). Excitation was achieved using a 295\u00a0nm LEDs ring lamp. Normalized fluorescence values were correlated with histological measurements using Pearson correlation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The UV fluorescence strongly exhibited a strong correlation with re\u2010epithelization (\n                      <jats:italic>r\u00a0<\/jats:italic>\n                      =\u00a00.8) at the wound edge, with peak intensity observed between the sixth and ninth days. Notably, wound\u2010healing dynamics differed between the wound center and edge, primarily attributed to variations in re\u2010epithelialization, neovascularization, and chronic inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings highlight the presence of autofluorescence at 295\/340\u00a0nm during wound healing, demonstrating a robust association with re\u2010epithelialization. This excitation\/emission signal holds promise as a valuable noninvasive strategy for monitoring wound closure, re\u2010epithelialization, and other biological processes where Tr plays a pivotal role.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.13834","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T08:23:14Z","timestamp":1719303794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tryptophan intrinsic fluorescence from wound healing correlates with re\u2010epithelialization in a rabbit model"],"prefix":"10.1111","volume":"30","author":[{"given":"Marco","family":"Duran\u2010Padilla","sequence":"first","affiliation":[{"name":"Pathology Department Hospital General de Mexico Dr. Eduardo Liceaga  Mexico City 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Rojas","sequence":"additional","affiliation":[{"name":"Teaching Department Hospital General de Mexico Dr. Eduardo Liceaga  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2060-9310","authenticated-orcid":false,"given":"Miguel","family":"Reyes\u2010Alberto","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Aplicadas y Tecnolog\u00eda (ICAT) Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walfre","family":"Franco","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Massachusetts Lowell  Lowell Massachusetts USA"},{"name":"Department of Dermatology University of Massachusetts Chan Medical School  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0571-2563","authenticated-orcid":false,"given":"Joselin","family":"Hernandez\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University of Utah  Salt Lake City Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enoch","family":"Gutierrez\u2010Herrera","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Aplicadas y Tecnolog\u00eda (ICAT) Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Mexico City 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