{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:03:18Z","timestamp":1792008198075,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005006","name":"Asan Institute for Life Sciences, Asan Medical Center","doi-asserted-by":"publisher","award":["2020IF0009"],"award-info":[{"award-number":["2020IF0009"]}],"id":[{"id":"10.13039\/501100005006","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005006","name":"Asan Institute for Life Sciences, Asan Medical Center","doi-asserted-by":"publisher","award":["2024IP0034"],"award-info":[{"award-number":["2024IP0034"]}],"id":[{"id":"10.13039\/501100005006","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The epidermal barrier acts as a defense against external agents as well as helps to maintain body homeostasis. Polynucleotides (PN), exogenous DNA fragments, promote wound repair through their stimulatory and anti\u2010inflammatory effects. Recent findings indicate a synergistic effect of PN and hyaluronic acid (HA) combinations in regulating inflammation and promoting cell proliferation. This study aims to elucidate the effects of PN and HA on repairing the epidermal barrier following its disruption by tape stripping (TS) in a mouse model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>After disrupting the epidermal barrier using TS, a formulation containing PN (14\u00a0mg\/mL) and HA (6\u00a0mg\/mL) was applied. Trans\u2010epidermal water loss (TEWL) was measured at 0, 3, 6, 24, 48, and 72\u00a0h. Mice were euthanized after the final application at 72\u00a0h, and tissue samples were analyzed for epidermal\/dermal thickness, neutrophil infiltration, and filaggrin expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We observed a significant reduction in TEWL in the PN+HA group compared to that in the control group (20.8\u00a0\u00b1\u00a00.5 vs. 43.7\u00a0\u00b1\u00a00.5\u00a0g\/m\n                      <jats:sup>2<\/jats:sup>\n                      h at 72\u00a0h,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), indicating an improvement in barrier function. Histological evaluation showed decreased epidermal and dermal thickening in the PN+HA group compared to that in the control group (epidermal: 29.4\u00a0\u00b1\u00a02.2 vs. 57.9\u00a0\u00b1\u00a03.5\u00a0\u03bcm; dermal: 464.8\u00a0\u00b1\u00a025.9 vs. 825.9\u00a0\u00b1\u00a044.8\u00a0\u03bcm, both\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Additionally, neutrophil infiltration in the dermis was significantly reduced, and filaggrin protein levels were significantly higher in the PN+HA group compared to those in the control group (4.8\u00a0\u00b1\u00a00.4 vs. 21.1\u00a0\u00b1\u00a03.3 for neutrophils; 0.84\u00a0\u00b1\u00a00.04 vs. 0.42\u00a0\u00b1\u00a00.03 for filaggrin, both\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These results suggest that PN+HA may be an effective therapeutic strategy for repairing skin barrier damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.70068","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T02:14:35Z","timestamp":1726798475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Effect of Polynucleotide\u2010Hyaluronic Acid Hydrogel in the Recovery After Mechanical Skin Barrier Disruption"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2001-7435","authenticated-orcid":false,"given":"Ye Jin","family":"Ha","sequence":"first","affiliation":[{"name":"Asan Institute for Life Sciences Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka Hee","family":"Tak","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences Asan Medical Center University of Ulsan College of Medicine  Seoul South 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Center University of Ulsan College of Medicine  Seoul South Korea"},{"name":"Division of Colon and Rectal Surgery Department of Surgery Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young\u2010Chang","family":"Ah","sequence":"additional","affiliation":[{"name":"BRPHARM Co., Ltd  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok\u2010Soon","family":"Kim","sequence":"additional","affiliation":[{"name":"BRPHARM Co., Ltd  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ik Jun","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Dermatology Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Sik","family":"Yoon","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"},{"name":"Division of Colon and Rectal Surgery Department of Surgery Asan Medical Center University of Ulsan College of Medicine  Seoul South 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