{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:45:04Z","timestamp":1753879504088,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007194","name":"Wenzhou Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["Y2023461"],"award-info":[{"award-number":["Y2023461"]}],"id":[{"id":"10.13039\/501100007194","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Adipose\u2010derived stem cells (ADSCs) exhibit good anti\u2010photoaging activity, yet inadequate homing numbers and low cell survival rates remain urgent challenges to overcome, and the underlying mechanisms are still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Methylcellulose (MC)\/hyaluronic acid (HA)\/Collagen I hydrogel (Gel) loaded with ADSCs (MHAC\/ADSCs Gel) was prepared using the thermal dispersion method. Scanning electron microscopy was used to observe its microstructure, while swelling and degradation rates were determined by the pycnometer method. Subsequently, a photoaging HaCat cell model was established to evaluate the anti\u2010photoaging potential of MHAC\/ADSCs using the CCK\u20108 assay and Transwell experiment. Furthermore, qRT\u2010PCR, WB, TargetScan bioinformatics analysis, and dual\u2010luciferase reporter assays were conducted to investigate the underlying mechanisms of MHAC\/ADSCs. Finally, a photoaging mouse model was established to assess the anti\u2010photoaging activity of MHAC\/ADSCs using H&amp;E staining, Masson staining, immunohistochemistry, qRT\u2010PCR, and WB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MHAC\/ADSCs exhibited a 3D porous structure, remaining in a sol state at 20<jats:sup>\u00b0<\/jats:sup>C and forming a gel at 37<jats:sup>\u00b0<\/jats:sup>C. Additionally, it demonstrated excellent swelling properties and degradability. The results also revealed that MHAC\/ADSCs showed significant anti\u2010photoaging activity, promoting the migration of HaCat cells, increasing the expression of miR\u201018a and Collagen I, and inhibiting the expression of HIF\u20101\u03b1. In animal experiments, MHAC\/ADSCs reversed epidermal thickening and collagen loss in mice and reduced the expression level of CD31.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MHAC\/ADSCs exhibit anti\u2010photoaging activity by inhibiting the expression of HIF\u20101\u03b1 through the upregulation of miR\u201018a, providing a promising approach for clinical treatment of photoaging.<\/jats:p><\/jats:sec>","DOI":"10.1111\/srt.70192","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T14:40:16Z","timestamp":1749739216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Injectable Methylcellulose\/Hyaluronic Acid\/Collagen Hydrogel Loaded With Adipose\u2010Derived Stem Cells Alleviates Skin Photoaging via Inhibition of HIF\u20101\u03b1: An in Vitro and in Vivo Study"],"prefix":"10.1111","volume":"31","author":[{"given":"Dashuai","family":"Li","sequence":"first","affiliation":[{"name":"Department of Stomatology The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4757-8727","authenticated-orcid":false,"given":"Pan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery The First Affiliated Hospital of Wenzhou Medical 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