{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:25:56Z","timestamp":1787660756031,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Skin aging is a complex process driven by multiplex factors, including chronic inflammation and UV exposure. It has become common practice for cosmetic formulations to counteract skin aging by targeting different pathways simultaneously with a pool of active ingredients. The present study aims to explore the skin protective effects of a novel extract of ferment in combination with copper tripeptide\u20101 GHK\u2010Cu from an anti\u2010inflammation perspective.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      An extract of the\n                      <jats:italic>Thermus thermophilus<\/jats:italic>\n                      and\n                      <jats:italic>Bacillus subtilis<\/jats:italic>\n                      mixed\u2010culture ferment (TBFE) was prepared and the skin protective effects of its combination with the human tripeptide derivative GHK\u2010Cu were explored through in vitro,\n                      <jats:italic>ex vivo<\/jats:italic>\n                      , and clinical tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The combination outperformed TBFE or GHK\u2010Cu alone in inhibiting secretion of inflammatory cytokines by lipopolysaccharide\u2010stimulated macrophages. In addition, one face cream formulated with the combination as the sole active ingredients protected human skin explants from ultraviolet irradiation induced damage by maintaining skin morphology and decreasing the degradation of extracellular matrix. Finally, results from the clinical study demonstrated that the combination was effective in decreasing wrinkles and improving skin barrier conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>It is concluded that the combination of TBFE and GHK\u2010Cu exerts anti\u2010aging effects on human skin by targeting inflammation, extracellular matrix, and skin barrier function. Therefore, the combination offers a promising option for anti\u2010aging skincare regimen by addressing multiple mechanisms involved in skin aging.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.70360","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T14:13:59Z","timestamp":1786371239000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Augmented Skin Beneficial Effects of\n                    <i>Thermus Thermophilus<\/i>\n                    and\n                    <i>Bacillus Subtilis<\/i>\n                    Mixed\u2010Culture Ferment Extract by Tripeptide GHK\u2010Cu"],"prefix":"10.1111","volume":"32","author":[{"given":"Jingyi","family":"Wang","sequence":"first","affiliation":[{"name":"Global R&amp;D Center Shanghai Chicmax Cosmetic Co. Ltd.  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