{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:02:10Z","timestamp":1784872930983,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Punch biopsies are considered the primary technique to obtain full\u2010thickness skin specimens for dermatologic diagnoses. However, limitations include scarring, sampling error, poor margin identification, and post\u2010sampling deformations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To introduce a novel micro\u2010biopsy technique utilizing a modified hypodermic needle to achieve full\u2010thickness skin micro\u2010columns and a streamlined post\u2010sampling process for histological analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ex\u2010vivo skin biopsies were taken using a modified hypodermic needle and compared to 3\u00a0mm punch biopsies. They were fixed and processed inside the needle, resulting in a firm, columnar sample, which was then sectioned, stained, and imaged. Dimensions and horizontal shrinkage were also calculated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The technique consistently yielded uniform, columnar\u2010shaped histological samples. These sections were of sufficient quality to perform staining with a significantly smaller horizontal shrinkage rate compared to standard 3\u00a0mm punch biopsies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We demonstrate a novel technique for a skin micro\u2010biopsy that allows for fixation and processing of the tissue inside the sampling instrument, yielding small, full\u2010thickness columnar skin histological samples that can be used for dermatological diagnosis with minimal post\u2010sampling deformation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Plain Language Summary<\/jats:title>\n                    <jats:p>Punch biopsies are considered the primary technique to obtain full\u2010thickness skin specimens in order to diagnose skin conditions. However, scarring associated with skin biopsies and the deformations that happen to samples during processing pose persistent challenges. To minimize invasiveness, sub\u2010millimeter skin punch biopsies have been introduced, but there has been no standardized, widely adoptable technique to utilize sub\u2010millimeter skin punch biopsies to make diagnoses. Here, we introduce a novel skin biopsy needle that obtains skin samples that are less than 0.5\u00a0mm in diameter, which is below the scarring threshold, as well as a processing technique to easily fix, stain, and image the samples to make skin diagnoses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.70362","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:52:12Z","timestamp":1783605132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microcore Skin Biopsies: A Novel, Micro\u2010Invasive Skin Biopsy Technique Yielding Full\u2010Thickness Histology"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5871-5680","authenticated-orcid":false,"given":"Rebecca","family":"Lapides","sequence":"first","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"},{"name":"The Robert Larner M.D. College of Medicine University of Vermont  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5696-4699","authenticated-orcid":false,"given":"Ga Ram","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenechi","family":"Iwelumo","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"},{"name":"Rutgers Robert Wood Johnson Medical School  New Brunswick New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Downs","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Sumner","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McCormack","sequence":"additional","affiliation":[{"name":"Division of Plastic &amp; Reconstructive Surgery Massachusetts General Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"William G.","family":"Austen","sequence":"additional","affiliation":[{"name":"Division of Plastic &amp; Reconstructive Surgery Massachusetts General Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Roider","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"},{"name":"Department of Dermatology University Hospital of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-4891","authenticated-orcid":false,"given":"Michael","family":"Wang\u2010Evers","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4508-045X","authenticated-orcid":false,"given":"Dieter","family":"Manstein","sequence":"additional","affiliation":[{"name":"Department of Dermatology Cutaneous Biology Research Center Harvard Medical School Massachusetts General Brigham Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.152519"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-2077.44174"},{"key":"e_1_2_11_4_1","first-page":"1155","article-title":"Punch Biopsy of the Skin","volume":"65","author":"Zuber T. 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