{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T08:46:20Z","timestamp":1782290780196,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Multiphoton tomography equipped with fluorescence lifetime imaging (MPT\u2010FLIM) is a novel noninvasive imaging technique for analyzing morphological and metabolic states of skin diseases at a subcellular resolution. The present study is the first to establish MPT\u2010FLIM as an imaging modality to monitor treatment response in psoriasis during topical anti\u2010inflammatory therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Patients with psoriasis treated with topical calcipotriol\/ betamethasone dipropionate (C\/B) or a calcipotriol derivative (C\/\u2010) formulation were recruited and monitored using MPT\u2010FLIM. Imaging was performed at baseline on day 0, and during treatment on days 3 and 28.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of six patients prescribed C\/B, six patients prescribed C\/\u2010 and four healthy controls were recruited. Characteristic histological features were visualized, including acanthosis, parakeratosis, papillomatosis, and thinning of the granular layer. There was a strong correlation between clinical, multiphoton tomographic, and pathophysiological improvement during treatment. Assessment of subclinical metabolic changes was a predictive parameter for treatment outcome. Detection of fluorescence signals from drug components allowed for tracking of drug distribution in intra\u2010 and intercellular spaces.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MPT\u2010FLIM proved to be a suitable tool for monitoring treatment response in psoriasis patients and tracking drug delivery. It could be a potential method for monitoring other inflammatory skin diseases during treatment to adjust therapy on an individual level.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/srt.70367","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:23:44Z","timestamp":1781238224000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intravital Monitoring of Psoriasis Treatment Response and Drug Delivery Using Multiphoton Fluorescence Lifetime Imaging"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1258-6390","authenticated-orcid":false,"given":"Lynhda","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8642-5226","authenticated-orcid":false,"given":"Christian","family":"Mess","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Herberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4679-7137","authenticated-orcid":false,"given":"Stefan W.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8988-1091","authenticated-orcid":false,"given":"Volker","family":"Huck","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61909-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61128-3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07405.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70112-X"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(03)01136-8"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000450826"},{"issue":"3","key":"e_1_2_10_8_1","article-title":"Topical Treatments for Chronic Plaque Psoriasis","volume":"2013","author":"Mason A. 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