{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:49:43Z","timestamp":1790804983054,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T00:00:00Z","timestamp":1354752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Antigens"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Abstract<\/jats:title>Bovine leukemia virus (BLV<\/jats:styled-content>) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Bovine leukocyte antigen (BoLA<\/jats:styled-content>) is strongly involved in the subclinical progression of BLV<\/jats:styled-content> infections. Recent studies show that the BoLA\u2010DRB3<\/jats:styled-content><\/jats:italic> gene might play a direct role in controlling the number of BLV<\/jats:styled-content>\u2010infected peripheral B lymphocytes in vivo<\/jats:italic> in Holstein cattle. However, the specific BoLA<\/jats:styled-content><\/jats:italic> class II<\/jats:styled-content> allele and DRB3\u2010DQA1<\/jats:styled-content><\/jats:italic> haplotypes determining the BLV<\/jats:styled-content> proviral load in Japanese Black cattle are yet to be identified. In this study, we focused on the association of BLV<\/jats:styled-content> proviral load and polymorphism of BoLA<\/jats:styled-content><\/jats:italic> class II<\/jats:styled-content> in Japanese Black cattle. We genotyped 186 BLV<\/jats:styled-content>\u2010infected, clinically normal cattle for BoLA\u2010DRB3<\/jats:styled-content><\/jats:italic> and BoLA\u2010DQA1<\/jats:styled-content><\/jats:italic> using a polymerase chain reaction\u2010sequence\u2010based typing method. BoLA\u2010DRB3<\/jats:styled-content>*0902<\/jats:italic> and BoLA\u2010DRB3<\/jats:styled-content>*1101<\/jats:italic> were associated with a low proviral load (LPVL), and BoLA\u2010DRB3<\/jats:styled-content>*1601<\/jats:italic> was associated with a high proviral load (HPVL). Furthermore, BoLA\u2010DQA1<\/jats:styled-content>*0204<\/jats:italic> and BoLA\u2010DQA1<\/jats:styled-content>*10012<\/jats:italic> were related to LPVL and HPVL, respectively. Furthermore, we confirmed the correlation between the DRB3\u2010DQA1<\/jats:styled-content><\/jats:italic> haplotype and BLV<\/jats:styled-content> proviral load. Two haplotypes, namely 0902B<\/jats:styled-content> or C (DRB3<\/jats:styled-content>*0902\u2010DQA1<\/jats:styled-content>*0204<\/jats:italic>) and 1101A<\/jats:styled-content> (DRB3<\/jats:styled-content>*1101\u2010DQA1<\/jats:styled-content>*10011<\/jats:italic>), were associated with a low BLV<\/jats:styled-content> proviral load, whereas one haplotype 1601B<\/jats:styled-content> (DRB3<\/jats:styled-content>*1601\u2010DQA1<\/jats:styled-content>*10012<\/jats:italic>) was associated with a high BLV<\/jats:styled-content> proviral load. We conclude that resistance is a dominant trait and susceptibility is a recessive trait. Additionally, resistant alleles were common between Japanese Black and Holstein cattle, and susceptible alleles differed. This is the first report to identify an association between the DRB3\u2010DQA1<\/jats:styled-content><\/jats:italic> haplotype and variations in BLV<\/jats:styled-content> proviral load.<\/jats:p>","DOI":"10.1111\/tan.12041","type":"journal-article","created":{"date-parts":[[2012,12,7]],"date-time":"2012-12-07T06:09:47Z","timestamp":1354860587000},"page":"72-82","source":"Crossref","is-referenced-by-count":89,"title":["Identification of bovine leukocyte antigen class II haplotypes associated with variations in bovine leukemia virus proviral load in Japanese Black cattle"],"prefix":"10.1111","volume":"81","author":[{"given":"T.","family":"Miyasaka","sequence":"first","affiliation":[{"name":"Viral Infectious Diseases Unit RIKEN Wako Saitama Japan"},{"name":"School of Veterinary Medicine Nihon University Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.\u2010n.","family":"Takeshima","sequence":"additional","affiliation":[{"name":"Viral Infectious Diseases Unit RIKEN Wako Saitama Japan"},{"name":"Laboratory of Viral Infectious Diseases Department of Medical Genome Sciences Graduate School of Frontier Science The University of Tokyo Wako Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jimba","sequence":"additional","affiliation":[{"name":"Viral Infectious Diseases Unit RIKEN Wako Saitama Japan"},{"name":"Laboratory of Viral Infectious Diseases Department of Medical Genome Sciences Graduate School of Frontier Science The University of Tokyo Wako Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Viral Infectious Diseases Unit RIKEN Wako Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Gifu Prefectural Livestock Research Institute Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Matsuhashi","sequence":"additional","affiliation":[{"name":"Gifu Prefectural Livestock Research Institute Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Sentsui","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Nihon University Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Aida","sequence":"additional","affiliation":[{"name":"Viral Infectious Diseases Unit RIKEN Wako Saitama Japan"},{"name":"Laboratory of Viral Infectious Diseases Department of Medical Genome Sciences Graduate School of Frontier Science The University of Tokyo Wako Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1128\/jvi.70.4.2178-2183.1996","article-title":"The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection","volume":"70","author":"Mirsky ML","year":"1996","journal-title":"J Virol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-4-18"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"6977","DOI":"10.4049\/jimmunol.151.12.6977","article-title":"Polymorphism in BoLA\u2010DRB3 exon 2 correlates with resistance to persistent lymphocytosis caused by bovine leukemia virus","volume":"151","author":"Xu A","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_7_5_1","first-page":"17","article-title":"Characterization and expression of bovine MHC class II genes","volume":"6","author":"Aida Y","year":"1995","journal-title":"Bull Soc Franco\u2010Japonais Sci Vet"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-7-91"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.16.1.593"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1999.tb01388.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2006.00332.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1169588"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050499"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050568"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.1.134"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-005-0045-6"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050268"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00179403"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2008.01092.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(02)74234-3"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(03)00744-3"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1999"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2005.01238.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.2007.00654.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2004.5.1.29"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002510000257"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1321-1329.2005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011-4621"},{"key":"e_1_2_7_27_1","first-page":"S12","article-title":"Influence of host genetic differences on leukemogenesis induced by bovine leukemia virus","volume":"17","author":"Aida Y","year":"2001","journal-title":"AIDS Res Hum Retroviruses"},{"key":"e_1_2_7_28_1","first-page":"337","article-title":"Association of BoLA class II haplotypes with subclinical progression of bovine leukaemia virus infection in Holstein\u2010Friesian cattle","volume":"27","author":"Zanotti M","year":"1996","journal-title":"Anim Genet"},{"key":"e_1_2_7_29_1","first-page":"383","article-title":"Genetic mechanisms of resistance and susceptibility to leukemia in Ayrshire and black pied cattle breeds determined by allelic distribution of gene Bola\u2010DRB3","volume":"39","author":"Udina IG","year":"2003","journal-title":"Genetika"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.1988.tb00800.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.1986.tb03191.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2008.01750.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2052.1998.00320.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.11.1220"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.1999.530401.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2002.590506.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-2-75"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(99)75443-3"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2052.2001.00774.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2010.10.007"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2011.01708.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2006.00751.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910190613"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910300517"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2008.01757.x"},{"key":"e_1_2_7_46_1","volume-title":"R: Language and Environment for Statistical Computing","author":"Team RDC","year":"2010"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.08.001"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5408.1748"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0997-11"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.12.7278"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2008.01140.x"},{"key":"e_1_2_7_52_1","first-page":"975","article-title":"Ovine MHC class II DRB1 alleles associated with resistance or susceptibility to development of bovine leukemia virus\u2010induced ovine lymphoma","volume":"59","author":"Nagaoka Y","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3848"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.3109\/13550289809114225"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04331-2"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1143"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.131"}],"container-title":["Tissue Antigens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftan.12041","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tan.12041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T05:59:51Z","timestamp":1700632791000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tan.12041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,6]]},"references-count":56,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/tan.12041"],"URL":"https:\/\/doi.org\/10.1111\/tan.12041","archive":["Portico"],"relation":{},"ISSN":["0001-2815","1399-0039"],"issn-type":[{"value":"0001-2815","type":"print"},{"value":"1399-0039","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,6]]}}}