{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:02Z","timestamp":1787016062225,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T00:00:00Z","timestamp":1379376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Antigens"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Abstract<\/jats:title>\n \n Next generation sequencing (NGS) is a promising technique that can reveal the entire gene sequences and to the highest possible resolution without any phase ambiguities. We have used this technique to investigate the frequencies of\n HLA<\/jats:styled-content>\n \u2010A, \u2010B, \u2010C, \u2010\n DRB1<\/jats:styled-content>\n and \u2010\n DQB1<\/jats:styled-content>\n in a Saudi cohort of healthy individuals. We used NGS using the 454 genome sequence (\n GS<\/jats:styled-content>\n )\n FLX<\/jats:styled-content>\n System and Conexio\n assign atf<\/jats:sc>\n 454 software to human leukocyte antigen (\n HLA<\/jats:styled-content>\n ) genotype eight class I and class\n II<\/jats:styled-content>\n loci. A total of 158 healthy Saudi adults were analyzed. The most frequently observed allele for\n HLA<\/jats:styled-content>\n \u2010A was\n \n HLA<\/jats:styled-content>\n \u2010A*02:01:01:01\n <\/jats:italic>\n (13.6%); for\n HLA<\/jats:styled-content>\n \u2010B,\n \n HLA<\/jats:styled-content>\n \u2010B*50:01:01\n <\/jats:italic>\n (15.8%); for\n HLA<\/jats:styled-content>\n \u2010C,\n \n HLA<\/jats:styled-content>\n \u2010C*06:02:01:01\n <\/jats:italic>\n (18.7%); for\n HLA\u2010DRB1<\/jats:styled-content>\n ,\n \n HLA\u2010DRB1<\/jats:styled-content>\n *07:01:01:01\n <\/jats:italic>\n (26.6%); and for\n HLA\u2010DQB1<\/jats:styled-content>\n ,\n \n HLA\u2010DQB1<\/jats:styled-content>\n *02:01:01\n <\/jats:italic>\n (20.3%). The most common four loci haplotypes in the Saudi population were\n \n HLA<\/jats:styled-content>\n \u2010A*24:02:01:01\u2010B*08:01:01\u2010C*07:02:01:01\u2010\n DRB1<\/jats:styled-content>\n *03:01:01:01\n <\/jats:italic>\n and\n \n HLA<\/jats:styled-content>\n \u2010A*23:01:01\u2010B*50:01:01\u2010C*06:02:01:01\u2010\n DRB1<\/jats:styled-content>\n *07:01:01:01\n <\/jats:italic>\n .. We have used a highly informative technique for\n HLA<\/jats:styled-content>\n typing of a Saudi healthy cohort to establish allele and haplotype frequencies. These results should prove useful for population studies, disease associations and future planning of the unrelated bone marrow donor registry.\n <\/jats:p>","DOI":"10.1111\/tan.12200","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T08:35:16Z","timestamp":1401957316000},"page":"252-258","source":"Crossref","is-referenced-by-count":34,"title":["HLA<\/scp>\n \u2010A, \u2010B, \u2010C, \u2010\n DRB1<\/scp>\n and \u2010\n DQB1<\/scp>\n allele and haplotype frequencies in Saudis using next generation sequencing technique"],"prefix":"10.1111","volume":"82","author":[{"given":"A. H.","family":"Hajeer","sequence":"first","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, King Abdulaziz Medical City National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"},{"name":"College of Medicine King Saud bin Abdulaziz University for Health Sciences, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Al Balwi","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, King Abdulaziz Medical City National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"},{"name":"College of Medicine King Saud bin Abdulaziz University for Health Sciences, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"},{"name":"King Abdullah International Medical Research Centre King Abdulaziz Medical City, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Ayt\u00fcl Uyar","sequence":"additional","affiliation":[{"name":"Department of Physiology, Istanbul Medical Faculty Istanbul University Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"AlHaidan","sequence":"additional","affiliation":[{"name":"King Abdullah International Medical Research Centre King Abdulaziz Medical City, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"AlAbdulrahman","sequence":"additional","affiliation":[{"name":"King Abdullah International Medical Research Centre King Abdulaziz Medical City, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Al Abdulkareem","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, King Abdulaziz Medical City National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"},{"name":"King Abdullah International Medical Research Centre King Abdulaziz Medical City, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Al Jumah","sequence":"additional","affiliation":[{"name":"College of Medicine King Saud bin Abdulaziz University for Health Sciences, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"},{"name":"King Abdullah International Medical Research Centre King Abdulaziz Medical City, National Guard Health Affairs Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,17]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"334","article-title":"HLA compatibility and organ transplant survival. 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