{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T23:34:15Z","timestamp":1715988855400},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Antigens"],"published-print":{"date-parts":[[2015,8]]},"abstract":"Abstract<\/jats:title>HLA<\/jats:styled-content>\u2010B*51<\/jats:italic>, a class I human leukocyte antigen (HLA<\/jats:styled-content>) molecule, is the strongest known genetic risk factor for Beh\u00e7et disease. However, there are only few articles reporting methods to determine the presence or absence of HLA\u2010B51<\/jats:styled-content>. For this reason, we designed and developed an easy, fast, and inexpensive real\u2010time high\u2010resolution melting (HRM<\/jats:styled-content>) assay to detect HLA<\/jats:styled-content>\u2010B*51<\/jats:italic>. We genotyped 61 samples by our HRM<\/jats:styled-content> assay and by conventional polymerase chain reaction, and no discrepancies were found between results. Besides, a subgroup of 25 samples was also genotyped in a different laboratory, and another subgroup of 16 samples was obtained from the International Histocompatibility Working Group DNA<\/jats:styled-content> Bank, and a full concordance of results was observed with those obtained by HRM<\/jats:styled-content>. Regarding the identifying system evaluated, we obtained 100% of specificity, sensibility, and repeatability, and 0% of false positive and false negative rates. Therefore, this HRM<\/jats:styled-content> analysis is easily applicable to the rapid detection of HLA<\/jats:styled-content>\u2010B*51<\/jats:italic>, exhibits a high speed, and requires a very low budget.<\/jats:p>","DOI":"10.1111\/tan.12603","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T17:23:40Z","timestamp":1436894620000},"page":"139-142","source":"Crossref","is-referenced-by-count":2,"title":["Rapid detection of HLA<\/scp>\u2010B*51<\/i> by real\u2010time polymerase chain reaction and high\u2010resolution melting analysis"],"prefix":"10.1111","volume":"86","author":[{"given":"C.","family":"Imperiali","sequence":"first","affiliation":[{"name":"Gen\u00e8tica Molecular\u2010Laboratori Cl\u00ednic, Hospital Universitari de Bellvitge L'Hospitalet de Llobregat Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Al\u00eda\u2010Ramos","sequence":"additional","affiliation":[{"name":"Gen\u00e8tica Molecular\u2010Laboratori Cl\u00ednic, Hospital Universitari de Bellvitge L'Hospitalet de Llobregat Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Padr\u00f3\u2010Miquel","sequence":"additional","affiliation":[{"name":"Gen\u00e8tica Molecular\u2010Laboratori Cl\u00ednic, Hospital Universitari de Bellvitge L'Hospitalet de Llobregat Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12107"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24642"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1407307111"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2002.590207.x"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406575111"},{"key":"e_1_2_3_7_1","unstructured":"IMGT\/HLA Database (ImMunoGeneTics project\/human major histocompatibility complex). Release 3.20.0 2015\u201004\u201017:http:\/\/www.ebi.ac.uk\/ipd\/imgt\/hla Last accessed: 2015\u201005\u201030."},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2001.580202.x"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.4238\/2014.July.2.8"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1998.tb03027.x"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2014.158"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4328"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2012.01942.x"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/gte.2005.9.292"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2001.580306.x"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1128"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2010.101"}],"container-title":["Tissue Antigens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftan.12603","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tan.12603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T23:33:27Z","timestamp":1696203207000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tan.12603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,14]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/tan.12603"],"URL":"https:\/\/doi.org\/10.1111\/tan.12603","archive":["Portico"],"relation":{},"ISSN":["0001-2815","1399-0039"],"issn-type":[{"value":"0001-2815","type":"print"},{"value":"1399-0039","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,14]]}}}