{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:51:58Z","timestamp":1790110318129,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T00:00:00Z","timestamp":1471996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2016YFC0905000"],"award-info":[{"award-number":["2016YFC0905000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China's Post-doctoral Science Fund","award":["2015M582695"],"award-info":[{"award-number":["2015M582695"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HLA"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human leukocyte antigen <jats:italic>HLA\u2010B*13:01<\/jats:italic> is identified currently as a marker of individual susceptibility to drug\u2010induced hypersensitivity reaction, such as dapsone\u2010induced hypersensitivity reactions (DIHRs) and trichloroethylene\u2010induced dermatitis. Therefore, screening for the <jats:italic>HLA\u2010B*13:01<\/jats:italic> allele can assist clinics in identifying patients at risk of developing DIHRs. By combining the allele\u2010specific primers with TaqMan probes, we established a single tube, triplex real\u2010time PCR to detect <jats:italic>HLA\u2010B*13:01<\/jats:italic>. The reliability of this assay was validated by the comparison of genotyping results with those by sequence\u2010based typing (SBT). With this assay, the distribution of <jats:italic>HLA\u2010B*13:01<\/jats:italic> in a total of 350 blood samples from four ethnic groups: Han, Tibetan, Uighur, and Buyei were determined. A 100% concordance was observed between the results with the established real\u2010time PCR and SBT in 100 samples. The detection limit of this assay was 0.016 ng genomic DNA. The prevalence of <jats:italic>HLA\u2010B*13:01<\/jats:italic> carriers were 11%, 8%, 1%, and 2% in the Buyei (<jats:italic>n<\/jats:italic> = 100), Northern Han (<jats:italic>n<\/jats:italic> = 100), Tibetan (<jats:italic>n<\/jats:italic> = 100), and Uighur (<jats:italic>n<\/jats:italic> = 50) populations, respectively. The multiplex real\u2010time PCR assay provided a fast and reliable method for accurate detection of <jats:italic>HLA\u2010B*13:01<\/jats:italic> allele prior to dapsone administration in clinical practice and onset of the reaction after exposure to trichloroethylene.<\/jats:p>","DOI":"10.1111\/tan.12863","type":"journal-article","created":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T02:33:10Z","timestamp":1472092390000},"page":"164-171","source":"Crossref","is-referenced-by-count":11,"title":["A multiplex allele\u2010specific real\u2010time polymerase chain reaction assay for <i>HLA\u2010B*13:01<\/i> genotyping in four Chinese populations"],"prefix":"10.1111","volume":"88","author":[{"given":"Z.","family":"Liu","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Miniaturized Detection Systems College of Life Science, Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Chen","sequence":"additional","affiliation":[{"name":"Gynecology Laboratory Xi an GaoXin Hospital  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Kang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Miniaturized Detection Systems College of Life Science, Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Han","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Miniaturized Detection Systems College of Life Science, Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Chen","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Miniaturized Detection Systems College of Life Science, Northwest University  Xi'an 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