{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T16:57:20Z","timestamp":1775581040861,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T00:00:00Z","timestamp":1506902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HLA"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>\n<jats:italic>HLA\u2010A*31:01<\/jats:italic> and <jats:italic>HLA\u2010B*15:02<\/jats:italic> have been widely reported to confer genetic susceptibility to carbamazepine (CBZ)\u2010induced severe cutaneous adverse reactions (SCARs). Accordingly, the screening for these alleles has been highly recommended to prevent SCAR prior to introducing CBZ therapy. Although a number of methods are available for screening of <jats:italic>HLA\u2010A*31:01<\/jats:italic> or <jats:italic>HLA\u2010B*15:02<\/jats:italic> alleles separately, developing an assay that can detect both these alleles would be more clinically practical, cost\u2010effective and less time\u2010consuming. Therefore, in this study, a multiplex polymerase chain reaction (PCR) using TaqMan Probe was designed and validated to be able to detect <jats:italic>HLA\u2010A*31:01<\/jats:italic> and <jats:italic>HLA\u2010B*15:02.<\/jats:italic> In comparison with Luminex\u2010SSO\/SBT\/SSB, the multiplex PCR assay for detection of <jats:italic>HLA\u2010A*31:01<\/jats:italic> and <jats:italic>HLA\u2010B*15:02<\/jats:italic> had a perfect agreement in the validation group of 125 samples. The method was able to detect the target genes at the DNA concentration of 0.037\u2009ng\/\u03bcL. The unit cost of this assay is less than $5 USD with total time of 110\u2009minutes.<\/jats:p>","DOI":"10.1111\/tan.13143","type":"journal-article","created":{"date-parts":[[2017,9,8]],"date-time":"2017-09-08T11:24:28Z","timestamp":1504869868000},"page":"335-342","source":"Crossref","is-referenced-by-count":18,"title":["A novel multiplex polymerase chain reaction assay for detection of both <i>HLA\u2010A*31:01<\/i>\/<i>HLA\u2010B*15:02<\/i> alleles, which confer susceptibility to carbamazepine\u2010induced severe cutaneous adverse reactions"],"prefix":"10.1111","volume":"90","author":[{"given":"D. V.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Sydney Medical School \u2013 Northern The University of Sydney  Sydney Australia"},{"name":"ImmunoRheumatology Laboratory, NSW Health Pathology\u2010North Royal North Shore Hospital  Sydney Australia"},{"name":"Department of Allergy and Clinical Immunology Hanoi Medical University  Hanoi Viet Nam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Vidal","sequence":"additional","affiliation":[{"name":"ImmunoRheumatology Laboratory, NSW Health Pathology\u2010North Royal North Shore Hospital  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. C.","family":"Chi","sequence":"additional","affiliation":[{"name":"Center of Allergology and Clinical Immunology Bach Mai Hospital  Hanoi Viet Nam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. T. 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