{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T20:27:32Z","timestamp":1784233652413,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>\n<jats:italic>HLA\u2010A*29<\/jats:italic> and <jats:italic>HLA\u2010B*51<\/jats:italic> are associated with birdshot uveitis and Beh\u00e7et's disease, respectively, and are used as a diagnostic criterion in patients with suspected disease, requiring their detection in diagnostic laboratories. While commercial tests for individual HLA alleles are available for other disease\u2010associated HLA variants, no similar allele\u2010specific assays are available for <jats:italic>HLA\u2010A*29<\/jats:italic> and <jats:italic>HLA\u2010B*51.<\/jats:italic> Here, we report sequence\u2010specific priming\u2010polymerase chain reaction (SSP\u2010PCR) methods for the detection of <jats:italic>HLA\u2010A*29<\/jats:italic> and <jats:italic>HLA\u2010B*51<\/jats:italic> using a single PCR reaction per allele. The assays were tested in 30 and 32 previously HLA\u2010typed samples, respectively, representing &gt;97% of <jats:italic>HLA\u2010A<\/jats:italic> alleles and &gt;93% of <jats:italic>HLA\u2010B<\/jats:italic> alleles in a European population. A concordance of 100% was observed with previous typing results, validating these methods for use in a diagnostic or research context.<\/jats:p>","DOI":"10.1111\/tan.13296","type":"journal-article","created":{"date-parts":[[2018,5,16]],"date-time":"2018-05-16T04:29:27Z","timestamp":1526444967000},"page":"90-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An SSP\u2010PCR method for the rapid detection of disease\u2010associated alleles <i>HLA\u2010A*29<\/i> and <i>HLA\u2010B*51<\/i>"],"prefix":"10.1111","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0880-456X","authenticated-orcid":false,"given":"U.","family":"Amstutz","sequence":"first","affiliation":[{"name":"University Institute of Clinical Chemistry, Center for Laboratory Medicine Inselspital Bern University Hospital, University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Schaerer","sequence":"additional","affiliation":[{"name":"University Institute of Clinical Chemistry, Center for Laboratory Medicine Inselspital Bern University Hospital, University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Andrey","sequence":"additional","affiliation":[{"name":"University Institute of Clinical Chemistry, Center for Laboratory Medicine Inselspital Bern University Hospital, University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Wirthmueller","sequence":"additional","affiliation":[{"name":"University Institute of Clinical Chemistry, Center for Laboratory Medicine Inselspital Bern University Hospital, University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. R.","family":"Largiad\u00e8r","sequence":"additional","affiliation":[{"name":"University Institute of Clinical Chemistry, Center for Laboratory Medicine Inselspital Bern University Hospital, University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,21]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00852.x"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0706135"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-19-88"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2016-309764"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24642"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123525"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.252"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2016.08.013"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.12.033"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.13143"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12603"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1161"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1166"},{"key":"e_1_2_2_15_1","unstructured":"JimKent.UCSC in\u2010Silico PCR.http:\/\/rohsdb.cmb.usc.edu\/GBshape\/cgi-bin\/hgPcr. 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