{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:02:25Z","timestamp":1747188145067,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T00:00:00Z","timestamp":1540857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["HHSN261200800001E"],"award-info":[{"award-number":["HHSN261200800001E"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>There are several aspects of HLA\u2010C gene expression that distinguish it from the HLA\u2010A and HLA\u2010B genes. First, HLA\u2010C is expressed by extravillous trophoblasts, whereas HLA\u2010A and HLA\u2010B are not. Second, its cell\u2010surface expression is much lower, which has been linked to changes in transcription and efficiency of peptide loading and export. Third, HLA\u2010C possesses a NK cell\u2010specific promoter and a complex alternative splicing system that regulates expression during NK cell development. In this study, we investigate the contribution of the HLA\u2010C core promoter to trophoblast\u2010specific expression. Analysis of transcription start sites showed the presence of a trophoblast\u2010associated start site and additional upstream TATA and CCAAT\u2010box elements in the HLA\u2010C promoter, suggesting the presence of an overlapping trophoblast\u2010specific promoter. A comparison of in vitro promoter activity showed that the HLA\u2010C promoter was more active in trophoblast cell lines than either the HLA\u2010A or HLA\u2010B promoters. Enhanced trophoblast activity was mapped to the central enhanceosome region of the promoter, and mutational analysis identified changes in the RFX\u2010binding region that generated a trophoblast\u2010specific enhancer.<\/jats:p>","DOI":"10.1111\/tan.13404","type":"journal-article","created":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T08:24:05Z","timestamp":1538382245000},"page":"288-297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of trophoblast\u2010specific elements in the HLA\u2010C core promoter"],"prefix":"10.1111","volume":"92","author":[{"given":"Jenna K.","family":"Johnson","sequence":"first","affiliation":[{"name":"Cancer and Inflammation Program Center for Cancer Research, National Cancer Institute  Frederick Maryland"},{"name":"University of Minnesota Medical Scientist Training Program  Minneapolis 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