{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T09:34:30Z","timestamp":1780479270938,"version":"3.54.1"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T00:00:00Z","timestamp":1632182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008223","name":"Universidade Federal do Paran\u00e1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008223","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>Human papillomavirus (HPV) infection is a necessary cause for cervical cancer (CC), but it also depends on genetic factors, such as HLA polymorphism. However, few reports addressed the role of amino acids residues at the HLA peptide\u2010binding cleft in HPV\u2010related cervical disease. Therefore, we aimed to investigate the association between<jats:italic>HLA\u2010B, HLA\u2010C,<\/jats:italic>and<jats:italic>HLA\u2010DRB1<\/jats:italic>polymorphism and amino acid residues composing the pockets of the peptide\u2010binding cleft of the respective polypeptide chains with cervical intraepithelial neoplasia (CIN II\/III). HLA typing was performed by PCR\u2010SSOP in 184 women with CIN II\/III and 174 controls from South Brazil. Associations were estimated by multivariate logistic regression. FDR test was performed to correct the<jats:italic>p<\/jats:italic>\u2010value for multiple comparisons.<jats:italic>HLA\u2010DRB1*13:01<\/jats:italic>was associated with protection against CIN II\/III, while<jats:italic>HLA\u2010C*03:04<\/jats:italic>was associated with susceptibility. The amino acid residues isoleucine, tyrosine, and leucine at positions 95, 116, and 163 of HLA\u2010C, respectively, were associated with CIN II\/III susceptibility. In contrast, serine at positions 11 and 13 of HLA\u2010DRB1 was associated with protection against the disease. Our results confirm previously reported associations between HLA and cervical diseases caused by HPV and suggest a role for amino acid residues at different positions of HLA\u2010C and HLA\u2010DRB1 in CIN II\/III. This finding may be further explored to better understand the genetic risk and the influence of immune response to CC development.<\/jats:p>","DOI":"10.1111\/tan.14429","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T10:49:48Z","timestamp":1631270988000},"page":"448-458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inside the pocket: Critical elements of<scp>HLA<\/scp>\u2010mediated susceptibility to cervical precancerous lesions"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6144-5471","authenticated-orcid":false,"given":"Let\u00edcia Boslooper","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Laboratory of Immunogenetics and Histocompatibility, Department of Genetics Universidade Federal do Paran\u00e1 (UFPR) Curitiba Brazil"},{"name":"Post\u2010graduation Program in Genetics, Department of Genetics Universidade 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Xavier","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics and Histocompatibility, Department of Genetics Universidade Federal do Paran\u00e1 (UFPR) Curitiba Brazil"},{"name":"Post\u2010graduation Program in Genetics, Department of Genetics Universidade Federal do Paran\u00e1 (UFPR) Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Newton S\u00e9rgio","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Post Graduate Program of Gynecology and Obstetrics, Hospital de Cl\u00ednicas, Universidade Federal do Paran\u00e1 (UFPR) Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0588-6096","authenticated-orcid":false,"given":"Maria da Gra\u00e7a","family":"Bicalho","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics and Histocompatibility, Department of Genetics Universidade Federal do 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