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This is the case of HP\u2010DM1 which was demonstrated by analysis of cell transfectants and epitope mapping to be exclusively KIR2DL1\u2010specific, covering all allotypes identified to date, except for KIR2DL1*022 and *020, and also to react with KIR2DS1*013. Here, we compared in immunofluorescence analyses the staining of HP\u2010DM1 with other available mAbs to precisely identify KIR2DL1<jats:sup>+<\/jats:sup> NK cells in potential donors for \u03b1\u03b2T\/B\u2010depleted haplo\u2010HSCT, with known <jats:italic>KIR<\/jats:italic> genotype. HP\u2010DM1 mAb was used in combination with EB6 or 11PB6 (anti\u2010KIR2DL1\/S1 and anti\u2010KIR2DL3*005), 143211 (anti\u2010KIR2DL1\/S5), and HP\u2010MA4 (anti\u2010KIR2DL1\/S1\/S3\/S5) mAbs, allowing the accurate identification of different KIR<jats:sup>+<\/jats:sup> NK cell subsets. These phenotypic evaluations appeared useful to dissect the expression pattern of various KIR2D in NK cells from <jats:italic>KIR2DL3*005<\/jats:italic><jats:sup>+<\/jats:sup> individuals, particularly if <jats:italic>KIR2DS1<\/jats:italic> is present. HP\u2010DM1 mAb remarkably refined NK cell phenotyping of donors carrying <jats:italic>KIR2DS5<\/jats:italic>, either in the centromeric or telomeric region. Functional assays with KIR2DL1<jats:sup>+<\/jats:sup>\/S1<jats:sup>+<\/jats:sup>\/S5<jats:sup>+<\/jats:sup> NK cells confirmed that only HP\u2010DM1 exclusively reacts with KIR2DL1. Finally, we demonstrated that HP\u2010DM1 mAb blocked KIR2DL1 recognition of C2<jats:sup>+<\/jats:sup> HLA\u2010C. Altogether, the data support that HP\u2010DM1 is a unique reagent valuable for characterizing KIR<jats:sup>+<\/jats:sup> NK cell subsets.<\/jats:p>","DOI":"10.1111\/tan.14640","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T16:48:00Z","timestamp":1650386880000},"page":"119-132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of <scp>KIR<\/scp><sup>+<\/sup><scp>NK<\/scp> cell subsets with a monoclonal antibody selectively recognizing <scp>KIR2DL1<\/scp> and blocking the specific interaction with <scp>HLA\u2010C<\/scp>"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0242-3157","authenticated-orcid":false,"given":"Raffaella","family":"Meazza","sequence":"first","affiliation":[{"name":"Laboratory of Immunology IRCCS Ospedale Policlinico San Martino  Genoa 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