{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:43:33Z","timestamp":1791776613655,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:p>The importance of anti\u2010HLA antibodies in transplantation settings, such as HLA\u2010mismatched or haploidentical hematopoietic stem cell transplantation and platelet refractoriness, is widely recognized. In previous reports, it was mentioned that several cut\u2010off values of donor\u2010specific anti\u2010HLA antibodies mean fluorescence intensity (MFI) were related to graft rejection in the environment of HLA mismatched stem cell transplantation and the aim of this study was to investigate the prevalence and risk factors of anti\u2010HLA antibodies according to those cut\u2010off values of MFI. A total of 3805 patients with hematologic disease were prospectively enrolled and analyzed. When using MFI of anti\u2010HLA antibodies \u2265500, \u22651000, \u22651500, \u22652000, \u22655000, and\u2009\u2265\u200910,000 as cut\u2010off values for positivity, the prevalence of class I or II anti\u2010HLA antibodies ranged from 4.6% to 20.2% in all cases. When the MFI cut\u2010off value was \u2265500 for positivity, multivariate analysis indicated that platelet transfusion, underlying disease, and pregnancy were the most important risk factors for the presence of anti\u2010HLA antibodies for the total patients. Subgroup analysis according to age, gender, and underlying disease showed that pregnancy was the most important risk factor for the presence of anti\u2010HLA antibodies. For all patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a03805), when anti\u2010HLA antibody positivity was defined according to different MFI cut\u2010off values, including \u22651000, \u22651500, \u22652000, \u22655000, and\u2009\u2265\u200910,000, an association of platelet transfusion and pregnancy with anti\u2010HLA antibodies was also demonstrated. Our results suggest that pregnancy and platelet transfusion are the main risk factors for the prevalence of anti\u2010HLA antibodies in haploid allograft candidates, providing evidence for guiding the evaluation of anti\u2010HLA antibodies and helping donor selection for HLA\u2010mismatched transplant candidates.<\/jats:p>","DOI":"10.1111\/tan.14704","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T04:53:15Z","timestamp":1654836795000},"page":"312-324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prevalence and risk factors of antibodies to <scp>HLA<\/scp> according to different cut\u2010off values of mean fluorescence intensity in haploidentical allograft candidates: A prospective study of 3805 subjects"],"prefix":"10.1111","volume":"100","author":[{"given":"Ning","family":"Ma","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation National Clinical Research Center for Hematologic Disease, Peking University People's Hospital, Peking University Institute of Hematology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Pei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences, Peking University. 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