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As every patient possesses a defined set of HLA molecules, only certain antigens can be presented on the cell surface. Thus, studying HLA type\u2010dependent antigen presentation can improve the understanding of variation in susceptibility to various diseases, including infectious diseases and cancer. In archival formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue, the HLA type is difficult to analyze because of fragmentation of DNA, hindering the application of commonly used assays that rely on long DNA stretches. Addressing these difficulties, we present a refined approach for characterizing presence or absence of <jats:italic>HLA\u2010A<\/jats:italic>*02, the most common HLA\u2010A allele in the Caucasian population, in archival samples. We validated our genotyping strategy in a cohort of 90 samples with HLA status obtained by an NGS\u2010based method. 90% (n\u00a0=\u00a081) of the samples could be analyzed with the approach. For all of them, the presence or absence of <jats:italic>HLA\u2010A<\/jats:italic>*02 alleles was correctly determined with the method, demonstrating 100% sensitivity and specificity (95% CI: 91.40%\u2013100% and 91.19%\u2013100%). Furthermore, we provide an example of application in an independent cohort of 73 FFPE microsatellite\u2010unstable (MSI) colorectal cancer samples. As MSI cancer cells encompass a high number of mutations in coding microsatellites, leading to the generation of highly immunogenic frameshift peptide antigens, they are ideally suited for studying relations between the mutational landscape of tumor cells and interindividual differences in the immune system, including the HLA genotype. Overall, our method can help to promote studying HLA type\u2010dependency during the pathogenesis of a wide range of diseases, making archival and historic tissue samples accessible for identifying <jats:italic>HLA\u2010A<\/jats:italic>*02 alleles.<\/jats:p>","DOI":"10.1111\/tan.14846","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T15:14:59Z","timestamp":1666019699000},"page":"24-33","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A simple approach for detecting <i>HLA\u2010A<\/i>*02 alleles in archival formalin\u2010fixed paraffin\u2010embedded tissue samples and an application example for studying cancer immunoediting"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6648-8828","authenticated-orcid":false,"given":"Johannes","family":"Witt","sequence":"first","affiliation":[{"name":"Department of Applied Tumor Biology Institute of 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