{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T18:52:24Z","timestamp":1777143144898,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>In an ineffective transfusion context, solid\u2010phase immunoassays using the Luminex platform for the detection and characterization of HLA antibodies are currently used to select HLA\u2010compatible platelet products. A new HLA antibody identification method, the HISTO SPOT\u00ae HLA AB test (BAG Health care GmbH, Lich, Germany), based on the detection of antibodies directed against a recombinant single antigen (SA) by colored spots detected by HISTO MATCH HLA AB module software, runs fully automated on the MR.SPOT\u00ae. The aim of this study was to compare the ability of the HISTO SPOT HLA AB and C1qScreen\u2122 (C1q SAB) assays with that of the Labscreen single antigen class I (OL SAB) assay to detect anti\u2010HLA class I antibodies in 56 serum samples from 54 platelet refractory acute myeloid leukemia patients who received HLA mismatch platelet concentrates at a single oncohematology center. In total, 1414 class I specificities, 433 HLA\u2010A and 981 HLA\u2010B, were detected by the OL SAB test. The mean fluorescence intensity (MFI) was &gt;5000 for 874 antigens and\u2009&lt;5000 for 655 antigens. The HISTO SPOT\u00ae HLA AB and C1q SAB tests identified 85% and 79% of OL SA\u2010detected antigens with an MFI\u2009&gt;5000, respectively, but did not identify 34% and 44% of OL SAB\u2010detected antigens, highlighting the lower sensitivity of these techniques. Interestingly, the donor\u2010specific antibodies (DSAs) identified by the HISTO SPOT\u00ae HLA AB and C1q SAB assays reacted against HLA mismatch platelet concentrates with the same specificity (86%) and positive predictive (77%) value as in the OL SAB test when the MFI threshold was &gt;2000 for DSA detection. Although the HISTO SPOT\u00ae HLA AB test is less sensitive than the OL SAB test, this test could be used for the selection of HLA\u2010compatible platelet products.<\/jats:p>","DOI":"10.1111\/tan.15276","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T13:18:49Z","timestamp":1699622329000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of <scp>HLA<\/scp> antibody identification methods for the selection of platelet products for <scp>HLA<\/scp>\u2010mediated platelet refractory patients"],"prefix":"10.1111","volume":"103","author":[{"given":"Pascal","family":"Pedini","sequence":"first","affiliation":[{"name":"Immunogenetic and Histocompatibility Laboratory EFS PACC  Marseille France"},{"name":"ADES UMR Aix Marseille Univ  Marseille 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