{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T19:35:21Z","timestamp":1754163321914,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In organ transplantation, immunological risk assessment uses cell crossmatch (XM) results, which can be altered by several drugs. We observed two recipients awaiting kidney and liver transplantation with positive flow cytometry XM (FCXM) on T\u2010cells in the absence of Donor Specific Antibodies (DSA). Both were treated with vedolizumab (VDZ) for an inflammatory bowel disease (IBD). VDZ is an IgG1 directed against integrin \u03b14\u03b27, expressed on T and B cell subsets, we therefore suspected that VDZ interfered with XM. We tested with Luminex screening and single antigen assays and with auto\u2010 and allo\u2010FCXM on different HLA\u2010typed cells several sera from the two recipients, collected before and during treatment with VDZ. To assess the intensity and the kinetics of the interference, the sera collected during VDZ treatment were from the induction and the maintenance phases at different times post\u2010injection. All sera were DSA\u2010negative with Luminex assays, indicating the absence of interference of VDZ with virtual XM. IgG allo\u2010FCXM on T cells was positive during the maintenance phase while it was randomly positive on B cells. On T cells, the ratio was between 1.6 and 4.4 times the negative control (positivity threshold of 1.5) for the two recipients, and did not depend on the time between collection and injection. Unique T cell populations express Integrin \u03b14\u03b27, then we did not observe a global shift for T cells in the presence of VDZ but a shift for a small subset which was sufficient to induce a positive FCXM. VDZ treatment did not interfere with complement\u2010dependent cytotoxicity XM or with IgM FCXM. Pronase treatment abrogated VDZ interference. In the event of unexpected positive FCXM on T cells in a recipient with IBD, the transplant team should seek VDZ treatment and interference.<\/jats:p>","DOI":"10.1111\/tan.70301","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T08:23:53Z","timestamp":1751012633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deciphering the Interference of the Anti\u2010Integrin \u03b14\u03b27 Vedolizumab With Cell Crossmatch"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1141-1417","authenticated-orcid":false,"given":"Marine","family":"Cargou","sequence":"first","affiliation":[{"name":"CHU de Bordeaux Laboratoire D'immunologie et Immunog\u00e9n\u00e9tique, H\u00f4pital Pellegrin  Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Lion","sequence":"additional","affiliation":[{"name":"CHU Amiens\u2010Picardie Site SUD Centre de Biologie Humaine, Service Histocompatibilit\u00e9  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Intissar","family":"Dalhoumi","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux Laboratoire D'immunologie et Immunog\u00e9n\u00e9tique, H\u00f4pital Pellegrin  Bordeaux France"},{"name":"Univ. Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, INSERM ERL 1303  Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elodie","family":"Wojciechowski","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux Laboratoire D'immunologie et Immunog\u00e9n\u00e9tique, H\u00f4pital Pellegrin  Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamy","family":"Ralazamahaleo","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux Laboratoire D'immunologie et Immunog\u00e9n\u00e9tique, H\u00f4pital Pellegrin  Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwendaline","family":"Guidicelli","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux Laboratoire D'immunologie et Immunog\u00e9n\u00e9tique, H\u00f4pital Pellegrin  Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2573-5989","authenticated-orcid":false,"given":"Nicolas","family":"Guillaume","sequence":"additional","affiliation":[{"name":"CHU Amiens\u2010Picardie Site SUD Centre de Biologie Humaine, Service Histocompatibilit\u00e9  Amiens France"},{"name":"EA4666 HEMATIM Jules Verne University of Picardie  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Visentin","sequence":"additional","affiliation":[{"name":"CHU de Bordeaux Laboratoire D'immunologie et Immunog\u00e9n\u00e9tique, H\u00f4pital Pellegrin  Bordeaux France"},{"name":"Univ. 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