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Using a murine\n Aire<\/jats:italic>\n \u2010deficient mTEC line (\n Aire<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n ), we previously reported that\n Aire<\/jats:italic>\n regulates cell adhesion genes, affecting homotypic mTEC\/mTEC and heterotypic mTEC\/thymocyte interaction in\u00a0vitro. Transcriptional analysis comparing\n Aire<\/jats:italic>\n \n WT<\/jats:italic>\n <\/jats:sup>\n with\n Aire<\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mTECs indicates that\n Aire<\/jats:italic>\n modulates antigen processing and presentation genes.\n Aire<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mTECs, but not\n Aire<\/jats:italic>\n \n WT<\/jats:italic>\n <\/jats:sup>\n , exhibited delayed expression of MHC II and CD74 molecules, suggesting that\n Aire<\/jats:italic>\n influences the MHC II presentation pathway. However,\n Aire<\/jats:italic>\n \n WT<\/jats:italic>\n <\/jats:sup>\n and\n Aire<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mTECs did not directly present the OVA\n 323\u2013339<\/jats:sup>\n peptide to OT\u2010II TCR transgenic single\u2010positive CD4+ T cells. When cocultured with splenocytes and dendritic cells, both mTEC types induce T cell activation and present the OVA\n 323\u2013339<\/jats:sup>\n peptide to OT\u2010II TCR transgenic T cells. To further understand\n Aire'<\/jats:italic>\n s role in MHC II antigen processing\/presentation, we evaluated the expression of the MHC II transactivator (\n Ciita<\/jats:italic>\n ), IFN\u2010\u03b3 receptors and IFN\u2010\u03b3\u2010induced GTPases, as well as lysosome and cathepsin L activities. After IFN\u2010\u03b3 treatment,\n Aire<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mTECs, but not\n Aire<\/jats:italic>\n \n WT<\/jats:italic>\n <\/jats:sup>\n , delayed the expression of\n Ciita<\/jats:italic>\n . Our results suggest\n that Aire<\/jats:italic>\n controls some steps of the MHC II antigen processing\/presentation\u2010related proteins.\n <\/jats:p>","DOI":"10.1111\/tan.70516","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T03:05:00Z","timestamp":1766459100000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Role of the Autoimmune Regulator Gene in the Control of\n MHC II<\/scp>\n Antigen\u2010Processing and Presentation by Medullary Thymic Epithelial Cells"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-0446","authenticated-orcid":false,"given":"A. C.","family":"Monteleone\u2010Cassiano","sequence":"first","affiliation":[{"name":"Basic and Applied Immunology Program, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. D.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Ferreirinha","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U. G.","family":"Cipriano","sequence":"additional","affiliation":[{"name":"Basic and Applied Immunology Program, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. F.","family":"Gembre","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeirao Preto Medical School University of Sao Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4189-2685","authenticated-orcid":false,"given":"V. L. D.","family":"Bonato","sequence":"additional","affiliation":[{"name":"Basic and Applied Immunology Program, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Department of Biochemistry and Immunology, Ribeirao Preto Medical School University of Sao Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4408-140X","authenticated-orcid":false,"given":"G. A.","family":"Passos","sequence":"additional","affiliation":[{"name":"Basic and Applied Immunology Program, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Department of Basic and Oral Biology, Ribeir\u00e3o Preto School of Dentistry University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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