{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:12:19Z","timestamp":1792793539445,"version":"4.5.0"},"reference-count":73,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bovine leukaemia virus (BLV) infects cattle, integrates into the host genome as a provirus, and induces a persistent infection that remains asymptomatic but can cause leukaemia\/lymphoma. Most BLV\u2010infected cell clones are created by massive depletion, and a few of these infected cell clones expand through the mitotic cycle, leading to the onset of lymphoma. Bovine lymphocyte antigen\n                    <jats:italic>(BoLA)\u2010DRB3<\/jats:italic>\n                    polymorphism is associated with susceptibility to BLV\u2010induced leukemogenesis. However, whether\n                    <jats:italic>BoLA\u2010DRB3<\/jats:italic>\n                    polymorphism affects the clonality of BLV\u2010infected cells in\u00a0vivo remains unknown. Here, we investigated whether lymphoma integration sites have specific features in cattle with varying susceptibility to lymphoma. Genomic DNA was extracted from 99 BLV\u2010infected Holstein cattle with lymphoma in a nationwide survey across Japan, and the integration sites were analysed using BLV proviral DNA\u2010capture sequencing, which we had previously developed. Among the integration sites identified in 99 animals, no identical sites were confirmed. Comparison of integration sites between cattle with susceptible and resistant\n                    <jats:italic>BoLA\u2010DRB3<\/jats:italic>\n                    alleles showed no significant differences in the distribution of integration sites on the chromosome and in the genes and intergenic regions. With respect to the orientation of the proviruses, or proviral structures between individuals with resistance or susceptibility to lymphoma. In contrast, resistant animals showed a significantly higher proportion of monoclonal cell types than susceptible animals. In summary, the\n                    <jats:italic>BoLA\u2010DRB3<\/jats:italic>\n                    polymorphism affects BLV clonality; for example, there is massive depletion and clonal expansion of infected cell clones in fully transformed clones obtained from BLV\u2010infected cattle with lymphoma.\n                  <\/jats:p>","DOI":"10.1111\/tan.70561","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T09:22:30Z","timestamp":1769592150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of\n                    <i>BoLA\u2010DRB3<\/i>\n                    Polymorphisms on Clonality of Bovine Leukaemia Virus\u2010Infected Cells of Cattle With Lymphoma"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0048-7212","authenticated-orcid":false,"given":"Noriko","family":"Fukushi","sequence":"first","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9357-8795","authenticated-orcid":false,"given":"Meripet Polat","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9487-9615","authenticated-orcid":false,"given":"Ryosuke","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8408-2622","authenticated-orcid":false,"given":"Sonoko","family":"Watanuki","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Saito","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0026-3888","authenticated-orcid":false,"given":"Yasunobu","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"},{"name":"Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"Support Unit for Bio\u2010Material Analysis, Research Resources Division RIKEN Center for Brain Science  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2712-1825","authenticated-orcid":false,"given":"Kazuyoshi","family":"Hosomichi","sequence":"additional","affiliation":[{"name":"Laboratory of Computational Genomics, School of Life Science Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1274-6606","authenticated-orcid":false,"given":"Jinliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Binzhou Animal Science and Veterinary Medicine Academy  Binzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9648-0480","authenticated-orcid":false,"given":"Shin\u2010nosuke","family":"Takeshima","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"},{"name":"Department of Food and Nutrition Jumonji University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7400-3587","authenticated-orcid":false,"given":"Yoko","family":"Aida","sequence":"additional","affiliation":[{"name":"Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"},{"name":"Shandong Binzhou Animal Science and Veterinary Medicine Academy  Binzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00328"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003646"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2222\/jsv.69.23"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003687"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12977\u2010022\u201000609\u20100"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705\u2010020\u201004945\u20104"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02595\u201022"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.25\u20100031"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01714\u201024"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01542\u201022"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/gm568"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15264"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.09.016"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201083909\u20103"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14050995"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010021\u201002307\u20100"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2057"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3084"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740\u20100929.2006.00332.x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/rbz4720150290"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740\u20100929.2009.00663.x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.03.005"},{"issue":"3","key":"e_1_2_11_24_1","first-page":"513","article-title":"Association 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L.","year":"2006","journal-title":"Genetics and Molecular Research"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgeb.2018.02.009"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.4314\/ovj.v10i3.12"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.630089"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12977\u2010019\u20100476\u2010z"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10101281"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12041"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12030352"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14285"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10050502"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12121451"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11020210"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102052.2008.01750.x"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.11606\/issn.1678\u20104456.bjvras.2017.123769"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.12566"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.17485\/ijst\/2018\/v11i24\/128164"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528\u2010016\u20109712\u20106"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14503"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1303"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20102427(95)05538\u2010X"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165\u20102427(96)05721\u20102"},{"issue":"3","key":"e_1_2_11_45_1","first-page":"344","article-title":"Point Mutation of p53 Tumor Suppressor Gene in Bovine Leukemia Virus\u2010Induced Lymphosarcoma","volume":"11","author":"Zhuang W.","year":"1997","journal-title":"Leukemia"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9051"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7569\u20107576.1998"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12060650"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9110909"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2019.08.015"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.09.026"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399\u20100039.2011.01708.x"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399\u20100039.2003.00080.x"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011\u20104621"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.12667\/mhc.29.158"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742\u20104690\u20107\u201091"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746\u20106148\u20108\u2010167"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348\u20100421.2003.tb03391.x"},{"issue":"4","key":"e_1_2_11_60_1","first-page":"975","article-title":"Ovine MHC Class II DRB1 Alleles Associated With Resistance or Susceptibility to Development of Bovine Leukemia Virus\u2010Induced Ovine Lymphoma","volume":"59","author":"Nagaoka Y.","year":"1999","journal-title":"Cancer Research"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201048899\u20104"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1169588"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010065X.1999.tb01388.x"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781780642215.0153"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251\u2010012\u20100612\u20106"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.70333"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.1.170"},{"key":"e_1_2_11_68_1","volume-title":"RNA Tumor Viruses. Supplements and Appendixes","author":"Weiss R.","year":"1985"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746\u20106148\u20106\u20101"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2015.08.020"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.01.002"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010012\u20101526\u2010y"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mgene.2013.12.005"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14120"}],"container-title":["HLA"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tan.70561","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tan.70561","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tan.70561","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T10:41:43Z","timestamp":1779360103000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tan.70561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,28]]},"references-count":73,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/tan.70561"],"URL":"https:\/\/doi.org\/10.1111\/tan.70561","archive":["Portico"],"relation":{},"ISSN":["2059-2302","2059-2310"],"issn-type":[{"value":"2059-2302","type":"print"},{"value":"2059-2310","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,28]]},"assertion":[{"value":"2025-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70561"}}