{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T09:08:43Z","timestamp":1780304923190,"version":"3.54.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["L244070"],"award-info":[{"award-number":["L244070"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Platelet transfusion refractoriness (PTR) is a major clinical challenge that increases bleeding risk and wastes blood resources. Immune\u2010mediated PTR is mainly caused by alloantibodies against HLA class I and human platelet antigens (HPA). To overcome this, transfusion of compatible platelets based on HLA cross\u2010reactive groups (CREGs) and HPA genotypes is considered effective; however, current detection methods remain costly, complex and time\u2010consuming, and no simple approach is available for direct CREGs detection. In this study, we developed a novel multiplex multicolour melting curve analysis (MMCA) assay that simultaneously detected HLA CREGs and the genotypes of 14 HPA systems (HPA\u20101\u20136, 9, 12, 13, 15, 21, 27, 30 and 31) within just six reaction tubes. Analytical validation demonstrated that the MMCA assay achieved an analytical sensitivity of 0.222\u20134.725\u2009ng\/\u03bcL, with excellent reproducibility, as indicated by intra\u2010assay coefficients of variation (CVs) of 0.07%\u20130.3% and inter\u2010assay CVs of 0.1%\u20130.6%, depending on the reaction. Clinical validation using 200 blood donor samples showed complete concordance with next\u2010generation sequencing (NGS) for CREGs detection and 95.5%\u2013100% concordance with a commercial kit for HPA genotyping. Sequencing further confirmed the superior accuracy of the MMCA assay, especially for the HPA\u20102, HPA\u20103 and HPA\u201015 systems, thereby effectively preventing misclassification. The newly developed MMCA assay provides rapid turnaround, simplicity and cost\u2010effectiveness. With high sensitivity, reproducibility and clinical accuracy, it offers a valuable tool for high\u2010throughput selection of HLA\u2010 and HPA\u2010matched platelets in immune\u2010mediated PTR and establishing platelet donor registries.<\/jats:p>","DOI":"10.1111\/tan.70626","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:08:37Z","timestamp":1772964517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid and Robust Multiplex Multicolour Melting Curve Analysis Enables Simultaneous Detection of\n                    <scp>HLA<\/scp>\n                    Cross\u2010Reactive Groups and\n                    <scp>HPA<\/scp>\n                    Genotypes"],"prefix":"10.1111","volume":"107","author":[{"given":"Huizhen","family":"Sun","sequence":"first","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Beijing Engineering Research Center of Laboratory Medicine  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Su","sequence":"additional","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Beijing Engineering Research Center of Laboratory Medicine  Beijing P. R. China"},{"name":"National Center for Clinical Laboratories, Peking Union Medical College Chinese Academy of Medical Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Shenzhen Blood Center  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Shenzhen Blood Center  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Chang","sequence":"additional","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Beijing Engineering Research Center of Laboratory Medicine  Beijing P. R. China"},{"name":"National Center for Clinical Laboratories, Peking Union Medical College Chinese Academy of Medical Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yan","sequence":"additional","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Beijing Engineering Research Center of Laboratory Medicine  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Ma","sequence":"additional","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"National Center for Clinical Laboratories, Peking Union Medical College Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Center of Biotherapy, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine Chinese Academy of Medical Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7790-1550","authenticated-orcid":false,"given":"Huimin","family":"Ji","sequence":"additional","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Beijing Engineering Research Center of Laboratory Medicine  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3163-7891","authenticated-orcid":false,"given":"Lunan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing P. R. China"},{"name":"Beijing Engineering Research Center of Laboratory Medicine  Beijing P. R. China"},{"name":"National Center for Clinical Laboratories, Peking Union Medical College Chinese Academy of Medical Sciences  Beijing P. R. 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