{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T05:03:05Z","timestamp":1779771785346,"version":"3.53.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC2167\/2\u2013390884018"],"award-info":[{"award-number":["EXC2167\/2\u2013390884018"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2024\u201002467"],"award-info":[{"award-number":["2024\u201002467"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007435","name":"\u00c5ke Wiberg Stiftelse","doi-asserted-by":"publisher","award":["M24\u20100004"],"award-info":[{"award-number":["M24\u20100004"]}],"id":[{"id":"10.13039\/100007435","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008738","name":"Jeanssons Stiftelser","doi-asserted-by":"publisher","award":["J2024\u20100002"],"award-info":[{"award-number":["J2024\u20100002"]}],"id":[{"id":"10.13039\/100008738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006285","name":"Magnus Bergvalls Stiftelse","doi-asserted-by":"publisher","award":["2024\u20101259"],"award-info":[{"award-number":["2024\u20101259"]}],"id":[{"id":"10.13039\/501100006285","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Killer cell immunoglobulin\u2010like receptors (KIR) and their cognate HLA ligands regulate the functions of natural killer (NK) cells. However, extensive sequence homology within the KIR family limits the ability of monoclonal antibodies to selectively recognise individual receptors, and no KIR2DS1\u2010specific reagent is available to date. Here, we comprehensively delineate the binding profile of the monoclonal antibody S22019F. Using Ba\/F3 cells, NK cell clones, and primary NK cells, we demonstrate that S22019F selectively binds KIR2DS1. Moreover, functional assays reveal that binding of S22019F to KIR2DS1 is preserved upon activation by its HLA\u2010C ligand. We further confirm that S22019F does not cross\u2010react to KIR2DL3*005 allotypes and we extend its application to the analysis of KIR2DS1\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Collectively, these findings underscore the value of S22019F as a reagent that selectively recognises KIR2DS1, enabling improved analyses of KIR2DS1\u2010bearing lymphocytes in fundamental and clinical research.\n                  <\/jats:p>","DOI":"10.1111\/tan.70748","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:09:17Z","timestamp":1778666957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibody\n                    <scp>S22019F<\/scp>\n                    Selectively Recognises\n                    <scp>KIR2DS1<\/scp>\n                    and Enables Analysis of\n                    <scp>KIR2DS1<\/scp>\n                    <sup>+<\/sup>\n                    <scp>NK<\/scp>\n                    Cells and T Cells"],"prefix":"10.1111","volume":"107","author":[{"given":"Eleni","family":"Bilev","sequence":"first","affiliation":[{"name":"Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet Karolinska University Hospital Huddinge  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Meinecke","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet Karolinska University Hospital Huddinge  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jascha","family":"Wienberg","sequence":"additional","affiliation":[{"name":"Institute of Medical Immunology Christian\u2010Albrechts\u2010University of Kiel and University Medical Center Schleswig\u2010Holstein  Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Boulouis","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet Karolinska University Hospital Huddinge  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Li","sequence":"additional","affiliation":[{"name":"BioLegend Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takatoku","family":"Oida","sequence":"additional","affiliation":[{"name":"BioLegend Inc.  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6948-7281","authenticated-orcid":false,"given":"Jakob","family":"Micha\u00eblsson","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet Karolinska University Hospital Huddinge  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Gustaf","family":"Ljunggren","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet Karolinska University Hospital Huddinge  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Bacher","sequence":"additional","affiliation":[{"name":"Institute of Medical Immunology Christian\u2010Albrechts\u2010University of Kiel and University Medical Center Schleswig\u2010Holstein  Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Wiethe","sequence":"additional","affiliation":[{"name":"BioLegend Inc.  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