{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T07:44:26Z","timestamp":1792395866726,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n The\n DRB4*01:03:01:02N<\/jats:italic>\n null allele, which abolishes surface expression of the DR53 molecule, has been primarily reported in association with the\n HLA\u2010DRB1*07~DQB1*03:03<\/jats:italic>\n (DQ9) haplotype. Its linkage with\n DRB1*04<\/jats:italic>\n remains poorly defined. In this study, 242 Spanish deceased donors carrying\n DRB1*04, DRB1*<\/jats:italic>\n 07, or\n DRB1*09<\/jats:italic>\n alleles were analysed to determine the distribution of\n DRB4*01:03:01:02\u2009N<\/jats:italic>\n and identify predictive haplotypes. HLA typing of loci\n A<\/jats:italic>\n ,\n B<\/jats:italic>\n ,\n C<\/jats:italic>\n ,\n DRB1<\/jats:italic>\n ,\n DQB1<\/jats:italic>\n , and\n DRB3\/4\/5<\/jats:italic>\n was performed using low\u2010resolution PCR\u2010SSP, PCR\u2010SSOP or PCR\u2010NGS methods accordingly, with specific amplification for\n DRB4*01:03:01:02N<\/jats:italic>\n . The null allele was detected in 17.2% of\n DRB1*07~<\/jats:italic>\n DQ9 and in 6.1% of\n DRB1*04<\/jats:italic>\n \u2010positive donors, exclusively in association with\n DRB1*04:02~<\/jats:italic>\n DQ8. Extended haplotype analysis revealed two combinations strongly linked to the null allele: H1 (\n HLA\u2010A*02:01~C*05~B*44:02~DRB1*04:02~<\/jats:italic>\n DQ8), present in 50% of null carriers, and H2 (\n HLA\u2010A*02:05~C*07~B*58~DRB1*04:02~<\/jats:italic>\n DQ8), consistently associated with the null allele (100%). These findings confirm the association between\n DRB4*01:03:01:02N<\/jats:italic>\n and\n DRB1*04:02<\/jats:italic>\n in Spain and identify two extended haplotypes\u2014particularly H2\u2014as novel predictive markers. Their recognition may improve the accuracy of HLA typing and support clinical decision\u2010making in transplantation settings.\n <\/jats:p>","DOI":"10.1111\/tan.70846","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T14:06:53Z","timestamp":1784210813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Two\n HLA<\/scp>\n Haplotypes Harbouring the\n DRB4*01:03:01:02N<\/i>\n Null Allele and\n DRB1*04:02~<\/i>\n DQ8<\/scp>\n in the Spanish Population"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3625-1851","authenticated-orcid":false,"given":"Cristina","family":"Abad\u2010Molina","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda e Inmunolog\u00eda Hospital Cl\u00ednico Universitario de Valladolid Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Boix","sequence":"additional","affiliation":[{"name":"Laboratorio de Histocompatibilidad Centro de Transfusi\u00f3n de la Comunidad Valenciana Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"C\u00e1rdaba\u2010Arranz","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda e Inmunolog\u00eda Hospital Cl\u00ednico Universitario de Valladolid Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Vilches\u2010Moreno","sequence":"additional","affiliation":[{"name":"UGC Hematolog\u00eda, Inmunolog\u00eda y Gen\u00e9tica Hospital Universitario Puerta del Mar C\u00e1diz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Bustamante\u2010Munguira","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Intensiva Hospital Cl\u00ednico Universitario de Valladolid Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Eiros\u2010Bouza","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda e Inmunolog\u00eda Hospital Cl\u00ednico Universitario de Valladolid Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando","family":"Coca\u2010Rojo","sequence":"additional","affiliation":[{"name":"Servicio de Nefrolog\u00eda Hospital Cl\u00ednico Universitario de Valladolid Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00352841"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.13178"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.13531"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.1999.540317.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2000.550106.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14762"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2021.04.004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12117"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2012.01917.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-02-481945"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2015.157"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajt.2022.11.013"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2003.00103.x"}],"container-title":["HLA"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tan.70846","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tan.70846","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tan.70846","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T07:21:32Z","timestamp":1792394492000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tan.70846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/tan.70846"],"URL":"https:\/\/doi.org\/10.1111\/tan.70846","archive":["Portico"],"relation":{},"ISSN":["2059-2302","2059-2310"],"issn-type":[{"value":"2059-2302","type":"print"},{"value":"2059-2310","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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