{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:55:44Z","timestamp":1790567744526,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To systematically analyse HLA\u2010A, \u2010B and \u2010C and human platelet antigen (HPA) genotypes of platelet donors in Jilin Province using next\u2010generation sequencing (NGS) technology, a comprehensive donor database was established. Additionally, potential novel alleles were identified, providing a scientific basis for enhancing the safety of clinical blood transfusions. DNA fragments from 200 platelet donor samples in Jilin Province were amplified using locus\u2010specific primers. Comprehensive sequencing of HLA and HPA genes was performed via NGS. Bioinformatics analysis was employed to process genotyping results and screen for novel genetic variants. Newly discovered alleles were validated by Sanger sequencing to ensure accuracy and reliability. HLA genotyping achieved three\u2010field allele resolution, revealing the highest\u2010frequency alleles are as follows:\n                    <jats:italic>HLA\u2010A*11:01:01<\/jats:italic>\n                    ,\n                    <jats:italic>HLA\u2010B*13:02:01<\/jats:italic>\n                    ,\n                    <jats:italic>HLA\u2010C*01:02:01<\/jats:italic>\n                    and\n                    <jats:italic>C*03:04:01<\/jats:italic>\n                    . A novel allele\n                    <jats:italic>B*49:91<\/jats:italic>\n                    (mutation: E2 24T&gt;C) was identified. For the HPA systems (HPA\u20101, \u20102, \u20103, \u20105, \u20106, \u201015, \u201021), high heterozygosity was observed in HPA\u20103 and HPA\u201015, while no bb homozygosity was detected in HPA\u20101, \u20102, \u20105, \u20106 or \u201021. The application of NGS in constructing a platelet HLA\/HPA gene database enables high\u2010resolution genotyping, laying a critical foundation for precise platelet matching. This significantly reduces the risk of platelet transfusion refractoriness (PTR) and facilitates the discovery of novel allelic variants. The database provides essential theoretical and practical guidance for future donor screening and personalised transfusion strategies.\n                  <\/jats:p>","DOI":"10.1111\/tan.70851","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:14:35Z","timestamp":1788873275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Next\u2010Generation Sequencing\u2010Based High\u2010Resolution Typing of\n                    <scp>HLA<\/scp>\n                    \u2010A, \u2010B, \u2010C and\n                    <scp>HPA<\/scp>\n                    Genes in Jilin Province: Building a Platelet Donor Database and Identifying Novel Alleles"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9434-7192","authenticated-orcid":false,"given":"Yu","family":"Han","sequence":"first","affiliation":[{"name":"Institute of Blood Transfusion, Changchun Blood Center  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