{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T07:44:38Z","timestamp":1792395878039,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100017399","name":"Bundesamt f\u00fcr Ausr\u00fcstung, Informationstechnik und Nutzung der Bundeswehr","doi-asserted-by":"publisher","award":["BAAINBw MHH\u2010HF\u2010ZB3\u20102024"],"award-info":[{"award-number":["BAAINBw MHH\u2010HF\u2010ZB3\u20102024"]}],"id":[{"id":"10.13039\/100017399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Severe thrombocytopenia and impaired platelet function remain major clinical challenges in hematologic diseases, chemotherapy, bone marrow failure, surgery and obstetric haemorrhage. Conventional platelet transfusions are limited by donor dependency, short shelf life, storage constraints, and immune\u2010mediated platelet refractoriness caused primarily by HLA incompatibility. Here, we present a scalable, donor\u2010independent platform to generate universally compatible, low\u2010immunogenic megakaryocytes (MKs) from induced pluripotent stem cells (iPSCs). Human iPSCs were genetically engineered by shRNA\u2010mediated silencing of \u03b22\u2010microglobulin (\u03b22M) and\/or class II transactivator (CIITA). Differentiation of HLA\u2010silenced iPSCs resulted in MKs with reduced HLA class I and II expression, capable to release functional platelets in\u00a0vitro and sustain platelet production in humanised mice with physiological distribution. To enhance product safety, gamma irradiation effectively eliminated proliferative contaminants while preserving MK function. This platform integrates immune engineering with scalable iPSC\u2010based manufacturing and safety optimization, addressing key limitations of conventional donor\u2010derived platelet products. Together, these findings demonstrate that HLA\u2010silenced iPSC\u2010derived MKs represent a promising off\u2010the\u2010shelf source of functional platelets with reduced immunogenicity. By combining donor\u2010independent production, targeted immune engineering, and scalable manufacturing, this promising approach has the potential to improve transfusion support, overcome platelet refractoriness associated with HLA incompatibility, and expand access to readily available platelet therapies across diverse clinical settings.<\/jats:p>","DOI":"10.1111\/tan.70870","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T01:40:41Z","timestamp":1784857241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Immunological Engineering Platform for Scalable Production of\n                    <scp>iPSC<\/scp>\n                    \u2010Derived Megakaryocytes"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8017-098X","authenticated-orcid":false,"given":"Rabea","family":"Dettmer","sequence":"first","affiliation":[{"name":"Institute 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