{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T09:44:06Z","timestamp":1792748646238,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HLA"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the performance and clinical applicability of a novel third\u2010generation sequencing (TGS) strategy for high\u2010resolution HLA class I genotyping, employing nanopore long\u2010read sequencing combined with a dedicated analytical workflow, HLATyper. A total of 49 clinical samples were analysed in parallel using conventional PCR\u2010based sequence\u2010based typing (PCR\u2010SBT) and a TGS\u2010based approach on the G\u2010seq500 platform with the in\u2010house\u2010developed HLATyper pipeline. Genotyping results for HLA\u2010A, \u2010B and \u2010C loci were compared between the two methods with respect to concordance, typing resolution and the ability to resolve ambiguous allele assignments. Ambiguity rates between the two methods were evaluated using Fisher's exact test. Complete concordance was observed between PCR\u2010SBT and the TGS method at the two\u2010field level across all samples. Ambiguous allele assignments were detected in 63.27% (31\/49) of cases using PCR\u2010SBT, whereas the TGS strategy produced a single, unambiguous genotype for every sample (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). Furthermore, TGS enabled assignment of four\u2010field typing through comprehensive sequence alignment and successfully resolved ambiguities and exonic coverage gaps inherent to short\u2010read approaches. G\u2010seq500 platform\u2010based nanopore long\u2010read sequencing combined with the HLATyper analytical pipeline enables high\u2010resolution, unambiguous and comprehensive HLA class I genotyping. This integrated strategy represents a valuable complementary approach for clinical applications requiring precise HLA matching, such as transplantation and platelet transfusion support.\n                  <\/jats:p>","DOI":"10.1111\/tan.71051","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T03:52:33Z","timestamp":1792381953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Resolution\n                    <scp>HLA<\/scp>\n                    Class I Genotyping Using G\u2010Seq Platform\u2010Based Nanopore Long\u2010Read Sequencing Integrated With the\n                    <scp>HLATyper<\/scp>\n                    Pipeline"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2538-0356","authenticated-orcid":false,"given":"Hong\u2010Hong","family":"He","sequence":"first","affiliation":[{"name":"Department of Transfusion Research Laboratory Suzhou Blood Center  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5925-5168","authenticated-orcid":false,"given":"Run\u2010Yu","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resources and Eco\u2010Environment of Ministry of Education, Southwest Bio\u2010resources R&amp;D Key Laboratory of Sichuan Province, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences, Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0344-707X","authenticated-orcid":false,"given":"Qiu\u2010Lei","family":"Zhong","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resources and Eco\u2010Environment of Ministry of Education, Southwest Bio\u2010resources R&amp;D Key Laboratory of Sichuan Province, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences, Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-1176","authenticated-orcid":false,"given":"Ming\u2010Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Transfusion Research Laboratory Suzhou Blood Center  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7494-5363","authenticated-orcid":false,"given":"Miao","family":"He","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8355-7255","authenticated-orcid":false,"given":"Long\u2010Hai","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Transfusion Research Laboratory Suzhou Blood Center  Suzhou Jiangsu 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