{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T05:21:20Z","timestamp":1784697680751,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":669,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transbound Emerg Dis"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1111\/tbed.12139","type":"journal-article","created":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T16:40:48Z","timestamp":1383928848000},"page":"119-124","source":"Crossref","is-referenced-by-count":15,"special_numbering":"supplement s2","title":["Development of a Real-Time PCR Assay for Detection and Quantification of<i>Anaplasma ovis<\/i>Infection"],"prefix":"10.1155","volume":"60","author":[{"given":"Q.","family":"Chi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"},{"name":"College of Veterinary Medicine; Xinjiang Agricultural University; Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Yue","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine; Xinjiang Agricultural University; Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Grazing Animal Diseases MOA; Key Laboratory of Veterinary Parasitology of Gansu Province; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,8]]},"reference":[{"key":"10.1111\/tbed.12139-BIB0001|tbed12139-cit-0001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1865-1682.2010.01173.x","article-title":"A review of bovine anaplasmosis","volume":"58","author":"Aubry","year":"2011","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1111\/tbed.12139-BIB0002|tbed12139-cit-0002","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1586\/14737159.4.4.559","article-title":"Molecular diagnosis of human granulocytic anaplasmosis","volume":"4","author":"Dumler","year":"2004","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1111\/tbed.12139-BIB0003|tbed12139-cit-0003","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.19.1.165-256.2006","article-title":"Real-time PCR in clinical microbiology: applications for a routine laboratory testing","volume":"19","author":"Espy","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1111\/tbed.12139-BIB0004|tbed12139-cit-0004","first-page":"99","article-title":"Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp","volume":"39","author":"Friedhoff","year":"1997","journal-title":"Parassitologia"},{"key":"10.1111\/tbed.12139-BIB0005|tbed12139-cit-0005","doi-asserted-by":"crossref","first-page":"381","DOI":"10.7589\/0090-3558-42.2.381","article-title":"Genetic characterization of Anaplasma ovis strains from bighorn sheep in Montana","volume":"42","author":"Fuente","year":"2006","journal-title":"J. Wildl. Dis."},{"key":"10.1111\/tbed.12139-BIB0006|tbed12139-cit-0006","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.vetmic.2006.09.011","article-title":"Sequence analysis of the msp4 gene of Anaplasma ovis strains","volume":"119","author":"Fuente","year":"2007","journal-title":"Vet. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0007|tbed12139-cit-0007","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1101\/gr.6.10.986","article-title":"Real time quantitative PCR","volume":"6","author":"Heid","year":"1996","journal-title":"Genome Res."},{"key":"10.1111\/tbed.12139-BIB0008|tbed12139-cit-0008","first-page":"567","article-title":"Anaplasmosis. VII. Experimental Anaplasma ovis infection in white-tailed deer (Dama virginiana)","volume":"24","author":"Krier","year":"1963","journal-title":"Am. J. Vet. Res."},{"key":"10.1111\/tbed.12139-BIB0009|tbed12139-cit-0009","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1128\/AEM.06848-11","article-title":"Molecular survey and genetic identification of Anaplasma species in goats from central and southern China","volume":"78","author":"Liu","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0010|tbed12139-cit-0010","doi-asserted-by":"crossref","first-page":"16s","DOI":"10.1007\/BF02632909","article-title":"Ovine anaplasmosis in northwest China","volume":"29","author":"Lu","year":"1997","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1111\/tbed.12139-BIB0011|tbed12139-cit-0011","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1128\/JCM.02536-10","article-title":"Development and evaluation of a loop-mediated isothermal amplification method for rapid detection of Anaplasma ovis","volume":"49","author":"Ma","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0012|tbed12139-cit-0012","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1128\/JCM.33.3.675-679.1995","article-title":"Detection of Anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay","volume":"33","author":"Ndung'u","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0013|tbed12139-cit-0013","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1128\/JCM.02405-09","article-title":"Detection of Anaplasma marginale and A.\u00a0phagocytophilum in bovine peripheral blood samples by duplex real-time reverse transcriptase PCR assay","volume":"48","author":"Reinbold","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0014|tbed12139-cit-0014","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1128\/JCM.27.12.2730-2735.1989","article-title":"Cloned DNA probes identify Anaplasma ovis in goats and reveal a high prevalence of infection","volume":"27","author":"Shompole","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0015|tbed12139-cit-0015","first-page":"487","article-title":"Anaplasma ovis in the United States; experimental studies with sheep and goats","volume":"17","author":"Splitter","year":"1956","journal-title":"Am. J. Vet. Res."},{"key":"10.1111\/tbed.12139-BIB0016|tbed12139-cit-0016","doi-asserted-by":"crossref","first-page":"5139","DOI":"10.1128\/IAI.60.12.5139-5144.1992","article-title":"The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species","volume":"60","author":"Visser","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1111\/tbed.12139-BIB0017|tbed12139-cit-0017","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1128\/JCM.00196-10","article-title":"Development of a real-time PCR assay for detecting and quantifying human bocavirus 2","volume":"49","author":"Xu","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1111\/tbed.12139-BIB0018|tbed12139-cit-0018","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.rvsc.2010.02.009","article-title":"Phylogenetic analysis of the genus Anaplasma in southwestern China based on 16S rRNA sequence","volume":"89","author":"Zhou","year":"2010","journal-title":"Res. Vet. Sci."}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftbed.12139","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1111\/tbed.12139\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T15:44:44Z","timestamp":1675957484000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tbed.12139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":18,"URL":"https:\/\/doi.org\/10.1111\/tbed.12139","relation":{},"ISSN":["1865-1674"],"issn-type":[{"value":"1865-1674","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]}}}