{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:28:30Z","timestamp":1775147310941,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T00:00:00Z","timestamp":1622246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K05977"],"award-info":[{"award-number":["18K05977"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transbounding Emerging Dis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe and Asia; however, no report of APPV in Japan has been published. We identified an APPV in the central nervous system of Japanese piglets with CT and firstly determined and analysed the complete genome sequence. Phylogenetic analysis using the complete genome nucleotide sequence of the Japanese APPV, named Anna\/2020, and those of APPVs from the NCBI database showed that APPVs were divided into three genotypes (genotypes 1 to 3), and that Anna\/2020 clustered with the genotype 3 APPV strains, but distantly branched from these strains. Pairwise complete coding region nucleotide sequence comparisons revealed that there was 94.0%\u2013 99.7% sequence identity among the genotype 3 strains, while Anna\/2020 showed 87.0%\u201389.3% identity to those genotype 3 strains, suggesting that Anna\/2020 represents a novel APPV lineage within genotype 3. Retrospective examinations using RT\u2010PCR revealed one genotype 1 and two novel genotype 3 APPVs from pigs without CT, and that novel genotype 3 APPVs have been prevalent in Japan since at least 2007.<\/jats:p>","DOI":"10.1111\/tbed.14149","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T10:51:26Z","timestamp":1620816686000},"page":"1761-1769","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan"],"prefix":"10.1155","volume":"69","author":[{"given":"Miwako","family":"Kasahara\u2010Kamiie","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Kagawa","sequence":"additional","affiliation":[{"name":"Kagawa Livestock Hospital Annaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Shiokawa","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science Nippon Veterinary and Life Science University Musashino Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fujiko","family":"Sunaga","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Fukase","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoyuki","family":"Aihara","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanori","family":"Shiga","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Kamiie","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Aoki","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science Nippon Veterinary and Life Science University Musashino Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5884-620X","authenticated-orcid":false,"given":"Makoto","family":"Nagai","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Azabu University Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150104"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12532"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.04167\u201013"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12854"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13154"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12060680"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00857\u201008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.104824"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/v8100271"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12981"},{"key":"e_1_2_9_12_1","first-page":"429","article-title":"First report of atypical porcine pestivirus in piglets with congenital tremor in Canada","volume":"59","author":"Dessureault F. 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