{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T10:41:13Z","timestamp":1786444873505,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T00:00:00Z","timestamp":1622764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transbounding Emerging Dis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A total of 200 samples from Porcine circovirus 2 suspected (<jats:italic>n<\/jats:italic> = 112) and healthy (<jats:italic>n<\/jats:italic> = 88) swine populations collected from different districts of Tamil Nadu, south India were used in this study. The samples comprising of serum (n = 124), swabs from natural orifices (<jats:italic>n<\/jats:italic> = 52), and postmortem tissues (<jats:italic>n<\/jats:italic> = 24). All the samples were processed and subjected to the screening and detection of the PCV2 genome by a specific PCR assay. PCV2 genomes from positive samples were further subjected to genotyping with specifically designed primers for the full\u2010length amplification of the <jats:italic>ORF2<\/jats:italic> gene which codes for capsid protein (Cp) and serves as an epidemiological marker. Randomly, 13 amplified <jats:italic>ORF2<\/jats:italic> genes were sequenced and the aligned sequences were subjected to signature motif analysis and phylogeny in MEGA X. The molecular prevalence of PCV2 infection in Tamil Nadu is 10.5% (<jats:italic>n<\/jats:italic> = 21). Signature motif and phylogenetic studies of 13 samples revealed 38.5% (<jats:italic>n<\/jats:italic> = 5) presence of each PCV2b intermediate 1(IM1) and PCV2b genotypes, followed by 15.4% (<jats:italic>n<\/jats:italic> = 2) PCV2d\u20102 and 7.7% (<jats:italic>n<\/jats:italic> = 1) PCV2d genotypes. The PCV2b\u2010IM1 genotype has a 99.43% sequence homology with Vietnam isolate (JX506730). PCV2b genotypes showed 99.72% sequence identity with Chinese isolate (KX068219). PCV2d\u20102 genotypes reported in this study have 100% sequence identity with Taiwan isolate (MF169721). PCV2d genotype showed 97.87% sequence identity with Thailand isolate (MF314293). Amino acid analysis of all the 13 full\u2010length ORF2 gene sequences revealed specific mutations in the immune reactive domains of A, B, C, and D. Capsid protein of three PCV2b and five PCV2b IM1 isolates had extra amino acid residue lysine (K) at 234 position of ORF2 similar to PCV2d. For the first time in South India, PCV2b IM1 and PCV2d\u20102 genotypes are reported. This study evidences the genetic shifts of PCV2 isolates in India and it is analogous to that of global genotypic shift.<\/jats:p>","DOI":"10.1111\/tbed.14158","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T03:44:15Z","timestamp":1621395855000},"page":"1804-1812","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Emergence of novel Porcine circovirus <i>2<\/i> genotypes in Southern India"],"prefix":"10.1155","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9852-4245","authenticated-orcid":false,"given":"Parthiban","family":"S","sequence":"first","affiliation":[{"name":"Vaccine Research Centre\u2010Viral vaccines CAHS MMC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh","family":"A","sequence":"additional","affiliation":[{"name":"Vaccine Research Centre\u2010Viral vaccines CAHS MMC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anbu Kumar","family":"Karuppannan","sequence":"additional","affiliation":[{"name":"Vaccine Research Centre\u2010Viral vaccines CAHS MMC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhinakar Raj","family":"G","sequence":"additional","affiliation":[{"name":"Director Centre for Animal Health Studies MMC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnson Rajeswar","family":"J","sequence":"additional","affiliation":[{"name":"Professor &amp; Head Dept. of VMC VCRI  Tirunelveli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemalatha","family":"S","sequence":"additional","affiliation":[{"name":"Professor Dept of VPP MVC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaisree","family":"S","sequence":"additional","affiliation":[{"name":"Assistant Professor Central University Laboratory CAHS MMC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthilkumar","family":"K","sequence":"additional","affiliation":[{"name":"Post Graduate Research Institute in Animal Sciences  Kattupakkam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balasubramanyam","family":"D","sequence":"additional","affiliation":[{"name":"Post Graduate Research Institute in Animal Sciences  Kattupakkam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parthiban","family":"M","sequence":"additional","affiliation":[{"name":"Professor &amp; Head Dept. of ABT MVC  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarathchandra","family":"Ghadevaru","sequence":"additional","affiliation":[{"name":"Dean Faculty of Basic Sciences MVC campus  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870001200102"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.01.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12977"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00087\u201015"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.08.014"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.10.012"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.10.005"},{"key":"e_1_2_9_9_1","unstructured":"Clark E. 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