{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:13:50Z","timestamp":1784117630783,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T00:00:00Z","timestamp":1622764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002865","name":"Chongqing Science and Technology Commission","doi-asserted-by":"publisher","award":["CSTC2018jcyjAX0372"],"award-info":[{"award-number":["CSTC2018jcyjAX0372"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002865","name":"Chongqing Science and Technology Commission","doi-asserted-by":"publisher","award":["CSTC2016jcyjA0387"],"award-info":[{"award-number":["CSTC2016jcyjA0387"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transbounding Emerging Dis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lumpy skin disease (LSD) is a devastating viral disease that occurs in cattle. In China, it was first detected in the Xin\u2010Jiang autonomous region, near the border with Kazakhstan, in August 2019. As there were no new occurrences of LSD in either country following the first detection, the initial introduction of the virus remains unknown. Arthropod vectors were considered as potential vectors. Consequently, to identify the arthropod vectors involved in transmitting LSD virus (LSDV), an insect surveillance campaign was launched at four different sites scattered along the border, and samples from 22 flying insect species were collected and subjected to PCR assays. Following the Agianniotaki LSDV vaccine and Sprygin's general LSDV assays, two kinds of non\u2010biting flies, namely, <jats:italic>Musca domestica L<\/jats:italic> and <jats:italic>Muscina stabulans<\/jats:italic>, were positive for LSDV. However, all the other insects tested negative. Viral DNA was only detected in wash fluid, implying body surface contamination of the virus. The negative test results suggest that non\u2010biting flies are the dominant insects involved in the observed local epidemic. Three genomic regions encoding <jats:italic>RPO30<\/jats:italic>, <jats:italic>GPCR<\/jats:italic>, and <jats:italic>LW126<\/jats:italic> were successfully sequenced and subjected to phylogenetic analysis. The sequences shared high homology with <jats:italic>LSDV\/Russia\/Saratov\/2017<\/jats:italic>, a recombinant vaccine\u2010like strain formerly identified in Russia, and clustered with LSDV vaccine strains in phylogenetic trees of <jats:italic>RPO30<\/jats:italic> and <jats:italic>LW126<\/jats:italic>. However, the <jats:italic>GPCR<\/jats:italic> gene was seen to be solely clustered with LSDV field strains, implying differences in host affinity between these closely related vaccine\u2010like strains. Despite this, there is no direct evidence to support cross\u2010border transmission of the vaccine\u2010like LSDV. To our knowledge, this is the first report of vaccine\u2010like LSDV DNA detection in non\u2010biting flies in China.<\/jats:p>","DOI":"10.1111\/tbed.14159","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T12:36:23Z","timestamp":1621946183000},"page":"1813-1823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Analysis of vaccine\u2010like lumpy skin disease virus from flies near the western border of China"],"prefix":"10.1155","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7418-0126","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Animal Center, Chongqing Key Laboratory of Pediatrics, and Ministry of Education Key Lab of Child Development and Disorders, and National Clinical Research Center for Child Health and Disorders, and China International Science and Technology Cooperation base of Child Development and Critical Disorders Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Animal Inspection and Quarantine Laboratory Technical Center of Chong\u2010Qing Custom  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Animal Center, Chongqing Key Laboratory of Pediatrics, and Ministry of Education Key Lab of Child Development and Disorders, and National Clinical Research Center for Child Health and Disorders, and China International Science and Technology Cooperation base of Child Development and Critical Disorders Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Animal Inspection and Quarantine Laboratory Technical Center of Chong\u2010Qing Custom  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meimei","family":"Shi","sequence":"additional","affiliation":[{"name":"Animal Inspection and Quarantine Laboratory Technical Center of Chong\u2010Qing Custom  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuping","family":"Nie","sequence":"additional","affiliation":[{"name":"Animal Inspection and Quarantine Laboratory Technical Center of Chong\u2010Qing Custom  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengfen","family":"Liu","sequence":"additional","affiliation":[{"name":"Animal Inspection and Quarantine Laboratory Technical Center of Chong\u2010Qing Custom  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengbao","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Quarantine Laboratory Technical Center of Yi\u2010Ning Custom  Yining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4149-7813","authenticated-orcid":false,"given":"Daochao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Animal Center, Chongqing Key Laboratory of Pediatrics, and Ministry of Education Key Lab of Child Development and Disorders, and National Clinical Research Center for Child Health and Disorders, and China International Science and Technology Cooperation base of Child Development and Critical Disorders Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Quarantine Laboratory Technical Center of Haikou Custom  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Lin","sequence":"additional","affiliation":[{"name":"Animal Quarantine Laboratory Technical Center of Chengdu Custom  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingguo","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Inspection and Quarantine Laboratory Technical Center of Chong\u2010Qing Custom  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.08.011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705\u2010020\u201004607\u20105"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104\u2010019\u20104412\u2010z"},{"issue":"22","key":"e_1_2_9_5_1","first-page":"549","article-title":"Clinical and pathological studies on lumpy skin disease in Egypt","volume":"127","author":"Ali A. A.","year":"1990","journal-title":"Veterinary Record"},{"key":"e_1_2_9_6_1","unstructured":"Amirgazin A. &Ragatova A.(2020 September 10).Lumpy skin disease virus strain KZ\u2010Kostanay\u20102018 partial genome (GenBank: MT992618.1).https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/MT992618.1"},{"key":"e_1_2_9_7_1","unstructured":"AU\u2010IBAR. 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Report presented at the ninth meeting of the Standing Group of Experts on Lumpy Skin Disease (SGE LSD9).https:\/\/web.oie.int\/RR-Europe\/eng\/Regprog\/docs\/docs\/LSD9\/LSD%20presentations\/KZ_LSD_PPT_SGE_LSD9_en.pdf"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12217"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12378"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2018.12.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705\u2010019\u201004229\u20106"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.09.038"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.010686\u20100"},{"issue":"01","key":"e_1_2_9_23_1","first-page":"1","article-title":"The first outbreak investigation of lumpy skin disease in China","volume":"37","author":"Liu P.","year":"2020","journal-title":"Animal Quarantine in China"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13261"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12624"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010021\u201002613\u20106"},{"issue":"2","key":"e_1_2_9_27_1","first-page":"422","article-title":"Real time PCR for the detection of field isolates of lumpy skin disease virus in clinical samples from cattle","volume":"53","author":"Pestova Y. 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