{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:35Z","timestamp":1787016095357,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T00:00:00Z","timestamp":1627344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transbounding Emerging Dis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pseudorabies (PR) is an acute infectious disease of pigs caused by pseudorabies virus (PRV), which has caused great economic losses to the pig industry worldwide. Reliable and timely diagnose is crucial for the surveillance, control and eradication of PR. Here, a real\u2010time fluorescent recombinase\u2010aided amplification (real\u2010time RAA) assay was established to detect PRV. Primers and probes were designed based on the conserved regions of the PRV\n                    <jats:italic>gE<\/jats:italic>\n                    gene. The assay was specific for the detection of wild\u2010type PRV, showing no cross\u2010reactivity with other important porcine viruses (including PRV\n                    <jats:italic>gE<\/jats:italic>\n                    \u2010deleted vaccine strains). Analytical sensitivity of the assay was three 50% tissue culture infectious doses (TCID\n                    <jats:sub>50<\/jats:sub>\n                    ) of PRV DNA per reaction with 95% reliability, which is comparable to that of a PRV\u2010specific real\u2010time PCR (qPCR) assay. In diagnosis of 206 clinical tissue samples, the diagnose accordance rate between the real\u2010time RAA assay and qPCR assay was 97.57% (201\/206). Interestingly, the amplified products of real\u2010time RAA could be visualized under a portable blue light instrument, making it possible for the rapid detection of PRV in resource\u2010limited settings and on\u2010site screening. Therefore, our developed real\u2010time RAA assay is a diagnostic method for the rapid detection of PRV in the field.\n                  <\/jats:p>","DOI":"10.1111\/tbed.14241","type":"journal-article","created":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T14:15:09Z","timestamp":1626531309000},"page":"2266-2274","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Detection of pseudorabies virus with a real\u2010time recombinase\u2010aided amplification assay"],"prefix":"10.1155","volume":"69","author":[{"given":"Fei","family":"Tu","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0134-2761","authenticated-orcid":false,"given":"Yongning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengkui","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintan","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinna","family":"Ge","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6451-0478","authenticated-orcid":false,"given":"Xin","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanchun","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine China Agricultural University  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.09.012"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2017.10.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/824630"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.03.028"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-019-1264-z"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2020.113904"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870802000405"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.07.018"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9070563"},{"issue":"1","key":"e_1_2_9_11_1","first-page":"11","article-title":"Evaluation 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