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In this study, we generated a recombinant rabies virus (RABV) expressing EG95 protein of <jats:italic>E. granulosus<\/jats:italic> (LBNSE\u2010EG95) as a bivalent candidate vaccine for use in sheep and cattle against CE and rabies, which is another severe health threat in CE\u2010endemic areas. It was found that EG95 was successfully expressed without altering the pathogenicity of parent LBNSE vector. Further study showed that LBNSE\u2010EG95 immunization in mice elicited activation of dendric cells (DCs) and B cells and induced Th1\u2010\/Th2\u2010mediated cellular immune responses, leading to robust production of RABV neutralizing antibodies and high level of EG95\u2010sepecific antibodies with more than 90% protection against CE. In addition, single dose of LBNSE\u2010EG95 conferred full protection against lethal RABV challenge in mice. Collectively, these results suggest that the recombinant LBNSE\u2010EG95 has the potential to be developed as an efficient bivalent vaccine for sheep and cattle use in endemic areas of CE and rabies.<\/jats:p>","DOI":"10.1111\/tbed.14292","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T15:54:10Z","timestamp":1629215650000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A recombinant rabies virus expressing <i>Echinococcus granulosus<\/i> EG95 induces protective immunity in mice"],"prefix":"10.1155","volume":"69","author":[{"given":"Tong","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Virology School of Public Health Cheeloo College of Medicine Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lele","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Virology School of Public 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