{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T13:58:54Z","timestamp":1782482334288,"version":"3.54.5"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073624"],"award-info":[{"award-number":["82073624"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transbounding Emerging Dis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, a total of 179,907 blood samples from populations with suspected <jats:italic>Brucella<\/jats:italic> spp. infections were collected between 2008 and 2020 and analyzed by the Rose Bengal plate test (RBPT) and serum agglutination test (SAT). Moreover, conventional biotyping, <jats:italic>B. abortus<\/jats:italic>\u2010<jats:italic>melitensis<\/jats:italic>\u2010<jats:italic>ovis<\/jats:italic>\u2010<jats:italic>suis<\/jats:italic> polymerase chain reaction (AMOS\u2010PCR), and multiple\u2010locus variable\u2010number tandem repeat analysis (MLVA) was applied to characterize the isolated strains. A total of 8103 (4.50%) samples were positive in RBPT, while 7705 (4.28%, 95% confidence interval (CI) 4.19\u20134.37) samples were positive in SAT. There was a significant difference in seroprevalence for human brucellosis over time, in different areas and different cities (districts) (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> = 2 = 32.23, 1984.14, and 3749.51, <jats:italic>p<\/jats:italic> &lt; .05). The highest seropositivity (8.22% (4, 965\/60393; 95% CI 8.00\u20138.44) was observed in Yulin City, which borders Inner Mongolia, Ningxia, and Gansu Province, China, regions that have a high incidence of human brucellosis. Moreover, 174 <jats:italic>Brucella<\/jats:italic> strains were obtained, including nine with <jats:italic>B. melitensis<\/jats:italic> bv. 1, 145 with <jats:italic>B. melitensis<\/jats:italic> bv. 3, and 20 with <jats:italic>B. melitensis<\/jats:italic> variants. After random selection, 132 <jats:italic>B. melitensis<\/jats:italic> were further genotyped using MLVA\u201016. The 132 strains were sorted into 100 MLVA\u201016 genotypes (GTs) (GT 1\u2013100), 81 of which were single GTs represented by singular independent strains. The remaining 19 shared GTs involved 51 strains, and each GT included two to seven isolates from the Shaan northern and Guanzhong areas. These data indicated that although sporadic cases were a dominant epidemic characteristic of human brucellosis in this province, more than 38.6% (51\/132) outbreaks were also found in the Shaan northern area and Guanzhong areas. The 47 shared MLVA\u201016 GTs were observed in strains (<jats:italic>n<\/jats:italic> = 71) from this study and strains (<jats:italic>n<\/jats:italic> = 337) from 19 other provinces of China. These data suggest that strains from the northern provinces are a potential source of human brucellosis cases in Shaanxi Province. It is urgent to strengthen the surveillance and control of the trade and transfer of infected sheep among regions.<\/jats:p>","DOI":"10.1111\/tbed.14320","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T17:43:42Z","timestamp":1631468622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Seroprevalence trend of human brucellosis and MLVA genotyping characteristics of <i>Brucella melitensis<\/i> in Shaanxi Province, China, during 2008\u20132020"],"prefix":"10.1155","volume":"69","author":[{"given":"Cui\u2010Hong","family":"An","sequence":"first","affiliation":[{"name":"Department of Plague and Brucellosis Shaanxi Center for Disease Control and Prevention  Xi'an China"},{"name":"Department of Microbiology and Immunology School of Medicine Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shou\u2010Min","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Plague and Brucellosis Shaanxi Center for Disease Control and Prevention  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang\u2010Xin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Plague and Brucellosis Shaanxi Center for Disease Control and Prevention  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suo\u2010Ping","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Plague and Brucellosis Shaanxi Center for Disease Control and Prevention  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u2010Yan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Plague and Brucellosis Shaanxi Center for Disease Control and Prevention  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjun","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention National Institute for Communicable Diseases Control and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4904-4878","authenticated-orcid":false,"given":"Zhi\u2010Guo","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention National Institute for Communicable Diseases Control and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Hui","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Plague and Brucellosis Shaanxi Center for Disease Control and Prevention  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen\u20102020\u2010039676"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104235"},{"key":"e_1_2_11_4_1","volume-title":"Techniques for the brucellosis laboratory","author":"Alton G. 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