{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:36:40Z","timestamp":1791956200302,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,9,26]],"date-time":"2012-09-26T00:00:00Z","timestamp":1348617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Multidrug\u2010resistant (MDR<\/jats:styled-content>) gram\u2010negative bacilli pose a serious and rapidly emerging threat to recipients of solid organ transplants (SOT<\/jats:styled-content>s). However, extended\u2010spectrum \u03b2\u2010lactamases (ESBL<\/jats:styled-content>s), as one of the cardinal mechanisms of resistance to antimicrobial agents in SOT<\/jats:styled-content> recipients, remain obscure. The aim of this study was to investigate the prevalence of strains producing ESBL<\/jats:styled-content>s in SOT<\/jats:styled-content> patients with MDR<\/jats:styled-content> gram\u2010negative bacilli infections and to identify the ESBL<\/jats:styled-content> genes carried by them.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>Isolates from various clinical specimens of SOT<\/jats:styled-content> recipients were identified using the VITEK<\/jats:styled-content> 2 system, and their antibiotic sensitivity was determined by the 2\u2010fold agar dilution method. Isolates suspected of producing ESBL<\/jats:styled-content> enzymes were tested by an ESBL<\/jats:styled-content> phenotypic confirmatory method, and the ESBL<\/jats:styled-content> genotypes were determined by the polymerase chain reaction and sequencing.<\/jats:p><\/jats:sec>Results<\/jats:title>A total of 80 MDR<\/jats:styled-content> gram\u2010negative strains were isolated from 350 SOT<\/jats:styled-content> recipients. Among these strains, 42 (52.5%) had an ESBL<\/jats:styled-content>\u2010positive phenotype; 33 (42.3%) patients with ESBL<\/jats:styled-content>\u2010positive infection were found. Molecular analysis showed that most of the isolates harbored bla<\/jats:italic>CTX<\/jats:styled-content>\u2010M\u20109 (78.6%), bla<\/jats:italic>TEM<\/jats:styled-content> (81.0%), and bla<\/jats:italic>SHV<\/jats:styled-content> (69.0%) genes. All the ESBL<\/jats:styled-content>\u2010producing strains were susceptible to carbapenems.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>MDR gram\u2010negative isolates from SOT<\/jats:styled-content> recipients are frequently ESBL<\/jats:styled-content> producers. TEM<\/jats:styled-content> and CTX<\/jats:styled-content>\u2010M9 were the predominant ESBL<\/jats:styled-content> types.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12001","type":"journal-article","created":{"date-parts":[[2012,9,27]],"date-time":"2012-09-27T15:56:02Z","timestamp":1348761362000},"page":"14-21","source":"Crossref","is-referenced-by-count":29,"title":["Prevalence of multidrug\u2010resistant gram\u2010negative bacilli producing extended\u2010spectrum \u03b2\u2010lactamases (ESBL<\/scp>s) and ESBL<\/scp> genes in solid organ transplant recipients"],"prefix":"10.1111","volume":"15","author":[{"given":"T.\u2010Y.","family":"Men","sequence":"first","affiliation":[{"name":"Department of Urology Shandong University Affiliated Shandong Province Qianfoshan Hospital Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010N.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Gu","sequence":"additional","affiliation":[{"name":"Departments of General Surgery Affiliated First People's Hospital Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.\u2010H.","family":"Xing","sequence":"additional","affiliation":[{"name":"Departments of General Surgery Affiliated First People's Hospital Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.\u2010H.","family":"Peng","sequence":"additional","affiliation":[{"name":"Departments of General Surgery Affiliated First People's Hospital Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Zhong","sequence":"additional","affiliation":[{"name":"Departments of General Surgery Affiliated First People's Hospital Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-009-9225-x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00824-09"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.10.019"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.20214"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/jlts.2001.20769"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01855.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.04.004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2008.03.002"},{"key":"e_1_2_6_10_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; 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