{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:08:10Z","timestamp":1791958090156,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T00:00:00Z","timestamp":1431993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["PZ00P3_154709"],"award-info":[{"award-number":["PZ00P3_154709"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In immunosuppressed hosts, rapid identification of microorganisms of bloodstream infections is crucial to ensuring effective antimicrobial therapy. Conventional culture requires up to 72\u00a0h from sample collection to pathogen identification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used the SepsiTyper Kit and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>; Microflex, Bruker) directly from positive blood culture (BacT\/<jats:styled-content style=\"fixed-case\">ALERT<\/jats:styled-content> 3D, <jats:styled-content style=\"fixed-case\">FN<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> vials; bioM\u00e9rieux) in comparison to standard culture methodology (<jats:styled-content style=\"fixed-case\">VITEK<\/jats:styled-content> 2; bioM\u00e9rieux) for species identification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 62 consecutive positive blood cultures from immunosuppressed patients (solid organ or hematopoietic transplant recipients, or with febrile neutropenia) were analyzed. Culture yielded gram\u2010negative bacteria (<jats:styled-content style=\"fixed-case\">GNB<\/jats:styled-content>) in 27\/62 (43.5%) and gram\u2010positive (<jats:styled-content style=\"fixed-case\">GPB<\/jats:styled-content>) in 35\/62 (56.5%) vials. For <jats:styled-content style=\"fixed-case\">GNB<\/jats:styled-content>, the predominant species identified by <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> and confirmed by <jats:styled-content style=\"fixed-case\">VITEK<\/jats:styled-content> were <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>scherichia coli<\/jats:italic> (16\/16 correctly identified) and <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>nterobacter cloacae<\/jats:italic> (4\/4), with a sensitivity and specificity of 92.6% and 100%, respectively. For <jats:styled-content style=\"fixed-case\">GPB<\/jats:styled-content>, predominant species were <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>taphylococcus aureus<\/jats:italic> (3\/3), coagulase\u2010negative staphylococci (12\/24), and <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>nterococcus faecium<\/jats:italic> (6\/6) with a sensitivity of 100%, 60%, and 100%, respectively. The median time from blood collection to species identification was 27.4\u00a0h with <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> identification and 46.6\u00a0h with conventional methodology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Using <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> directly from positive blood cultures allowed a shorter time to identification with high sensitivity and specificity in immunosuppressed patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12373","type":"journal-article","created":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T15:27:07Z","timestamp":1424359627000},"page":"481-487","source":"Crossref","is-referenced-by-count":25,"title":["Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (<scp>MALDI<\/scp>\u2010<scp>TOF<\/scp>) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts"],"prefix":"10.1111","volume":"17","author":[{"given":"A.","family":"Egli","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology University Hospital Basel Basel Switzerland"},{"name":"Vaccine Research Infection Biology Lab Department Biomedicine University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Osthoff","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Goldenberger","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology University Hospital Basel Basel 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