{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:46:51Z","timestamp":1791269211832,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T00:00:00Z","timestamp":1440633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2015,10]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>This study aimed to characterize the dynamics of acquisition of cytomegalovirus (CMV<\/jats:styled-content>)\u2010specific cell\u2010mediated immunity (CMI<\/jats:styled-content>) in CMV<\/jats:styled-content> donor positive\/recipient negative solid organ transplant (SOT<\/jats:styled-content>) patients receiving long\u2010term antiviral prophylaxis, and to determine whether development of CMI<\/jats:styled-content> confers protection against CMV<\/jats:styled-content> disease.<\/jats:p><\/jats:sec>Methods<\/jats:title>A prospective multicenter study was conducted in Spain from September 2009 to September 2012. Whole blood specimens were prospectively collected at 30, 90, 120, 200, and 365\u00a0days after SOT<\/jats:styled-content>, and CMI<\/jats:styled-content> was determined by enumeration of CMV<\/jats:styled-content> pp65 and IE<\/jats:styled-content>\u20101\u2010specific CD<\/jats:styled-content>69+<\/jats:sup>\/interferon\u2010\u03b3\u2010producing CD<\/jats:styled-content>8+<\/jats:sup> and CD<\/jats:styled-content>4+<\/jats:sup> T cells by flow cytometry for intracellular cytokine staining. As part of a simultaneous clinical trial, patients received either early prophylaxis (in the first 3\u00a0days after transplantation) in the first period of the study or delayed prophylaxis (initiated at day 14) during the second period of the study. The impact of the dynamics of acquisition of CMV<\/jats:styled-content>\u2010specific CMI<\/jats:styled-content> on the incidence of CMV<\/jats:styled-content> disease was evaluated by Kaplan\u2013Meier survival analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>A total of 95 SOT<\/jats:styled-content> recipients were recruited. CMV<\/jats:styled-content> infection and disease occurred in 38 (40%) and 26 (27.4%) patients, respectively. The proportion of patients achieving any detectable CMV<\/jats:styled-content>\u2010specific CMI<\/jats:styled-content> response at each of the different monitoring points was higher in liver transplant recipients, as compared to kidney or heart transplant recipients. The presence of any detectable response at day 120 or 200 was protective against the development of CMV<\/jats:styled-content> disease (positive predictive values 92% and 93%, respectively).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The rate of acquisition of CMV<\/jats:styled-content>\u2010specific CMI<\/jats:styled-content> in SOT<\/jats:styled-content> recipients undergoing antiviral prophylaxis differed significantly between different SOT<\/jats:styled-content> populations. Patients developing any detectable CMI<\/jats:styled-content> response were protected against the occurrence of CMV<\/jats:styled-content> disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12417","type":"journal-article","created":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T07:11:04Z","timestamp":1435821064000},"page":"637-646","source":"Crossref","is-referenced-by-count":31,"title":["Effect of long\u2010term prophylaxis in the development of cytomegalovirus\u2010specific T\u2010cell immunity in D+\/R\u2212 solid organ transplant recipients"],"prefix":"10.1111","volume":"17","author":[{"given":"R.","family":"San\u2010Juan","sequence":"first","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario 12 de Octubre Instituto de Investigaci\u00f3n Hospital 12 de Octubre (i+12) Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Navarro","sequence":"additional","affiliation":[{"name":"Department of Microbiology Hospital Cl\u00ednico Universitario Instituto de Investigaci\u00f3n Sanitaria INCLIVA Universidad de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Garc\u00eda\u2010Reyne","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario 12 de Octubre Instituto de Investigaci\u00f3n Hospital 12 de Octubre (i+12) Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Montejo","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario de Cruces Bilbao Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n Madrid 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INCLIVA Universidad de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Gimenez","sequence":"additional","affiliation":[{"name":"Department of Microbiology Hospital Cl\u00ednico Universitario Instituto de Investigaci\u00f3n Sanitaria INCLIVA Universidad de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Eworo","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Sabe","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Hospital Universitari de Bellvitge IDIBELL Universitat de Barcelona Barcelona 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