{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T17:02:47Z","timestamp":1765040567054},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T00:00:00Z","timestamp":1460592000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003514","name":"Kommunfullm\u00e4ktige, Stockholms Stad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003514","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BK<\/jats:styled-content> polyomavirus (<jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV) can cause hemorrhagic cystitis (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>) in allogeneic hematopoietic stem cell transplant (allo\u2010<jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>) patients and polyomavirus\u2010associated nephritis in renal transplant patients, while <jats:styled-content style=\"fixed-case\">JC<\/jats:styled-content> polyomavirus (<jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV) can generate progressive multifocal leukoencephalopathy in immunocompromised individuals. Since 2007, additional human polyomaviruses (<jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yVs) have been identified. In this study, we examined the urines of allo\u2010<jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> patients for possible presence of polyomaviruses <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV, <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV, <jats:styled-content style=\"fixed-case\">KIP<\/jats:styled-content>yV, <jats:styled-content style=\"fixed-case\">WUP<\/jats:styled-content>yV, <jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>yV, <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yV6, <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yV7, <jats:styled-content style=\"fixed-case\">TSP<\/jats:styled-content>yV, <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yV9, and <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yV10 (<jats:styled-content style=\"fixed-case\">MWP<\/jats:styled-content>yV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 185 urinary samples obtained 2002\u20132007 from 105 allo\u2010<jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> patients, 32\/105 with <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>, were tested for the above\u2010listed <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yVs by a bead\u2010based multiplex assay. Of these, 142 urine samples had previously been tested for <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV and <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV by nested polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Aside from <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV and <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV, which dominated, <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yV7 was detected in 5 <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV\u2010positive urinary samples from 1 patient. The multiplex assay was more sensitive and specific than the nested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV and\/or <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV were found in all but 1 of the previously <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV\u2010 or <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV\u2010positive samples, although 6 previously <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV\u2010positive cases were now <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV\u2010positive or the reverse. Furthermore, 18\/79 previously negative samples were found to be <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV and\/or <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV positive, and a total of 21 double infections were found. Lastly, in 1\/29 <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content> patients, only <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV was detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>yV7 was found for the first time in urine of an allo\u2010<jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> patient, and <jats:styled-content style=\"fixed-case\">BKP<\/jats:styled-content>yV and <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV were more commonly found in urine samples using the bead\u2010based assay compared to testing by nested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Finally, only <jats:styled-content style=\"fixed-case\">JCP<\/jats:styled-content>yV was detected in the urine of 1 <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content> patient.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12500","type":"journal-article","created":{"date-parts":[[2016,1,26]],"date-time":"2016-01-26T10:58:51Z","timestamp":1453805931000},"page":"240-246","source":"Crossref","is-referenced-by-count":13,"title":["Studies of human polyomaviruses, with <scp>HP<\/scp>yV7, <scp>BKP<\/scp>yV, and <scp>JCP<\/scp>yV present in urine of allogeneic hematopoietic stem cell transplanted patients with or without hemorrhagic cystitis"],"prefix":"10.1111","volume":"18","author":[{"given":"J.","family":"Franz\u00e9n","sequence":"first","affiliation":[{"name":"Department of Oncology\u2010Pathology Karolinska Institutet Karolinska University Hospital Stockholm 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