{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:36:36Z","timestamp":1762252596193,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T00:00:00Z","timestamp":1506556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Saliva samples could be used for follow\u2010up of herpesviruses infection in pediatric transplant recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>With the aim of determining the frequency of herpesviral infections in saliva samples after transplantation, and the association with viremia and complications, a pilot longitudinal follow\u2010up of pediatric Cuban transplanted recipients (kidney and liver) was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative real\u2010time polymerase chain reaction of cytomegalovirus (<jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content>), Epstein\u2010Barr virus, herpes simplex virus, human herpesevirus\u20106 (<jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>6), varicella zoster virus, and human herpesvirus\u20108 were serially assayed in saliva and serum samples from 27 transplanted patients, during 32\u00a0weeks after the graft. Samples taken immediately after the graft were used as control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Herpesviruses were detected in 88.9% of saliva and in 37.0% of serum samples. <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>6 and <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> were the viruses more frequently detected (70.4%) in saliva and they were significantly more frequent during the follow\u2010up in comparison with control samples (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Most patients (22\/27) had more than one virus shedding concurrently. Patients with <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> in saliva were associated with <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> viremia (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.009), particularly at the cutoff of 252.5 copies\/mL, with a less accurate level of area under the curve. No association between <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> viral load in saliva and viral disease or response to the antiviral treatment was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The association found between <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> shedding in saliva and <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> viremia in this study opens the possibility of future studies of using viral load in saliva as a predictor of viremia. The implementation of herpesviral load in saliva samples for early clinical intervention in pediatric recipients needs a study with a large number of samples for further conclusions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12771","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T14:12:37Z","timestamp":1503324757000},"source":"Crossref","is-referenced-by-count":8,"title":["Herpesviruses excretion in saliva of pediatric transplant recipients"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2897-8388","authenticated-orcid":false,"given":"Consuelo Beatriz","family":"Correa Sierra","sequence":"first","affiliation":[{"name":"Sexually Transmitted Diseases Laboratory Virology Department Institute of Tropical Medicine \u201cPedro Kour\u00ed\u201d  Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian","family":"Kour\u00ed 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